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PDBsum entry 2ko0
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Transcription/DNA
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PDB id
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2ko0
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References listed in PDB file
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Key reference
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Title
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Structural determinants of specific DNA-Recognition by the thap zinc finger.
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Authors
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S.Campagne,
O.Saurel,
V.Gervais,
A.Milon.
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Ref.
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Nucleic Acids Res, 2010,
38,
3466-3476.
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PubMed id
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Abstract
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Human THAP1 is the prototype of a large family of cellular factors sharing an
original THAP zinc-finger motif responsible for DNA binding. Human THAP1
regulates endothelial cell proliferation and G1/S cell-cycle progression,
through modulation of pRb/E2F cell-cycle target genes including rrm1. Recently,
mutations in THAP1 have been found to cause DYT6 primary torsion dystonia, a
human neurological disease. We report here the first 3D structure of the complex
formed by the DNA-binding domain of THAP1 and its specific DNA target (THABS)
found within the rrm1 target gene. The THAP zinc finger uses its double-stranded
beta-sheet to fill the DNA major groove and provides a unique combination of
contacts from the beta-sheet, the N-terminal tail and surrounding loops toward
the five invariant base pairs of the THABS sequence. Our studies reveal
unprecedented insights into the specific DNA recognition mechanisms within this
large family of proteins controlling cell proliferation, cell cycle and
pluripotency.
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