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PDBsum entry 2kh9
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References listed in PDB file
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Key reference
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Title
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Structure and functional implications of a complex containing a segment of u6 RNA bound by a domain of prp24.
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Authors
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S.Martin-Tumasz,
N.J.Reiter,
D.A.Brow,
S.E.Butcher.
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Ref.
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Rna, 2010,
16,
792-804.
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PubMed id
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Abstract
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U6 RNA plays a critical role in pre-mRNA splicing. Assembly of U6 into the
spliceosome requires a significant structural rearrangement and base-pairing
with U4 RNA. In the yeast Saccharomyces cerevisiae, this process requires the
essential splicing factor Prp24. We present the characterization and structure
of a complex containing one of Prp24's four RNA recognition motif (RRM) domains,
RRM2, and a fragment of U6 RNA. NMR methods were used to identify the preferred
U6 binding sequence of RRM2 (5'-GAGA-3'), measure the affinity of the
interaction, and solve the structure of RRM2 bound to the hexaribonucleotide
AGAGAU. Interdomain contacts observed between RRM2 and RRM3 in a crystal
structure of the free protein are not detectable in solution. A structural model
of RRM1 and RRM2 bound to a longer segment of U6 RNA is presented, and a partial
mechanism for Prp24's annealing activity is proposed.
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