PDBsum entry 2k4r

Hydrolase

PDB id: 2k4r

Contents

Protein chain

77 a.a.

References listed in PDB file

Key reference

• Title: Nmr solution structure of the neurotrypsin kringle domain.
• Authors: O.A.Ozhogina, A.Grishaev, E.L.Bominaar, L.Patthy, M.Trexler, M.Llinás.
• Ref.: Biochemistry, 2008, 47, 12290-12298.
• PubMed id: 18956887

Abstract

Neurotrypsin is a multidomain protein that serves as a brain-specific serine protease. Here we report the NMR structure of its kringle domain, NT/K. The data analysis was performed with the BACUS (Bayesian analysis of coupled unassigned spins) algorithm. This study presents the first application of BACUS to the structure determination of a 13C unenriched protein for which no prior experimental 3D structure was available. NT/K adopts the kringle fold, consisting of an antiparallel beta-sheet bridged by an overlapping pair of disulfides. The structure reveals the presence of a surface-exposed left-handed polyproline II helix that is closely packed to the core beta-structure. This feature distinguishes NT/K from other members of the kringle fold and points toward a novel functional role for a kringle domain. Functional divergence among kringle domains is discussed on the basis of their surface and electrostatic characteristics.