UniProt functional annotation for P22646



	UniProt functional annotation for P22646

UniProt code: P22646.

Organism: Mus musculus (Mouse).

Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus.

Function: Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR- mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development (PubMed:19956738, PubMed:24899501). Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region. {ECO:0000250|UniProtKB:P07766, ECO:0000269|PubMed:19956738, ECO:0000269|PubMed:24470497, ECO:0000269|PubMed:24899501, ECO:0000269|PubMed:9843989}.

Subunit: The TCR-CD3 complex is composed of a CD3D/CD3E and a CD3G/CD3E heterodimers that preferentially associate with TCRalpha and TCRbeta, respectively, to form TCRalpha/CD3E/CD3G and TCRbeta/CD3G/CD3E trimers. In turn, the hexamer interacts with CD3Z homodimer to form the TCR-CD3 complex. Alternatively, TCRalpha and TCRbeta can be replaced by TCRgamma and TCRdelta. Interacts with CD6. Interacts with NCK1 (PubMed:24470497). {ECO:0000250|UniProtKB:P07766, ECO:0000269|PubMed:24470497}.

Subcellular location: Cell membrane {ECO:0000269|PubMed:24470497}; Single-pass type I membrane protein {ECO:0000250|UniProtKB:P07766}.

Ptm: Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8. {ECO:0000250|UniProtKB:P07766}.

Disruption phenotype: Absence of CD3E leads to the complete absence of mature T-cells. Thymocyte development is arrested at the early double- negative (DN) stage. {ECO:0000269|PubMed:9843989}.

Annotations taken from UniProtKB at the EBI.


Organism:		Mus musculus (Mouse).
Taxonomy:		Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus.



Function:		Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR- mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development (PubMed:19956738, PubMed:24899501). Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region. {ECO:0000250\|UniProtKB:P07766, ECO:0000269\|PubMed:19956738, ECO:0000269\|PubMed:24470497, ECO:0000269\|PubMed:24899501, ECO:0000269\|PubMed:9843989}.


Subunit:		The TCR-CD3 complex is composed of a CD3D/CD3E and a CD3G/CD3E heterodimers that preferentially associate with TCRalpha and TCRbeta, respectively, to form TCRalpha/CD3E/CD3G and TCRbeta/CD3G/CD3E trimers. In turn, the hexamer interacts with CD3Z homodimer to form the TCR-CD3 complex. Alternatively, TCRalpha and TCRbeta can be replaced by TCRgamma and TCRdelta. Interacts with CD6. Interacts with NCK1 (PubMed:24470497). {ECO:0000250\|UniProtKB:P07766, ECO:0000269\|PubMed:24470497}.
Subcellular location:		Cell membrane {ECO:0000269\|PubMed:24470497}; Single-pass type I membrane protein {ECO:0000250\|UniProtKB:P07766}.
Ptm:		Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8. {ECO:0000250\|UniProtKB:P07766}.
Disruption phenotype:		Absence of CD3E leads to the complete absence of mature T-cells. Thymocyte development is arrested at the early double- negative (DN) stage. {ECO:0000269\|PubMed:9843989}.