UniProt functional annotation for P56254



	UniProt functional annotation for P56254

UniProt code: P56254.

Organism: Oldenlandia affinis.

Taxonomy: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Gentianales; Rubiaceae; Rubioideae; Spermacoceae; Hedyotis-Oldenlandia complex; Oldenlandia.

Function: Probably participates in a plant defense mechanism. Has antibiotic activity. Has a diuretic effect. Has a uterotonic effect in humans. Active against the Gram-positive S.aureus with a minimum inhibition concentration of approximately 0.2 microM. Relatively ineffective against Gram-negative bacteria such as E.coli and P.aeruginosa. Inhibitory effect on the growth and development of larvae from H.punctigera. The unmodified form has hemolytic activity, the oxidized form lacks hemolytic activity. If the protein is linearized, hemolytic activity is lost. {ECO:0000255|PROSITE-ProRule:PRU00395, ECO:0000269|PubMed:12779323, ECO:0000269|PubMed:17534989}.

Tissue specificity: Leaves and stems. Lower in roots.

Domain: The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.

Ptm: Kalata-B1 is a cyclic peptide which occurs in three forms: with unmodified Trp-111, with Trp-111 oxidized to form oxindolylalanine and with Trp-111 oxidized to form N-formylkynurenine. Oxidation is enhanced by exposure to sunlight. {ECO:0000269|PubMed:17534989}.

Mass spectrometry: Mass=2892; Method=Electrospray; Evidence={ECO:0000269|PubMed:7703226};

Mass spectrometry: Mass=2982.4; Method=Electrospray; Evidence={ECO:0000269|PubMed:17534989};

Mass spectrometry: Mass=2908.4; Method=Electrospray; Note=With oxindolylalanine.; Evidence={ECO:0000269|PubMed:17534989};

Mass spectrometry: Mass=2924.4; Method=Electrospray; Note=With N- formylkynurenine.; Evidence={ECO:0000269|PubMed:17534989};

Pharmaceutical: The uteroactive properties of Kalata have been discovered by African traditional medicine. It is used as an ingredient of a herbal tea to accelerate childbirth.

Similarity: Belongs to the cyclotide family. Moebius subfamily. {ECO:0000255|PROSITE-ProRule:PRU00395}.

Annotations taken from UniProtKB at the EBI.


Organism:		Oldenlandia affinis.
Taxonomy:		Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Gentianales; Rubiaceae; Rubioideae; Spermacoceae; Hedyotis-Oldenlandia complex; Oldenlandia.



Function:		Probably participates in a plant defense mechanism. Has antibiotic activity. Has a diuretic effect. Has a uterotonic effect in humans. Active against the Gram-positive S.aureus with a minimum inhibition concentration of approximately 0.2 microM. Relatively ineffective against Gram-negative bacteria such as E.coli and P.aeruginosa. Inhibitory effect on the growth and development of larvae from H.punctigera. The unmodified form has hemolytic activity, the oxidized form lacks hemolytic activity. If the protein is linearized, hemolytic activity is lost. {ECO:0000255\|PROSITE-ProRule:PRU00395, ECO:0000269\|PubMed:12779323, ECO:0000269\|PubMed:17534989}.


Tissue specificity:		Leaves and stems. Lower in roots.
Domain:		The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.
Ptm:		Kalata-B1 is a cyclic peptide which occurs in three forms: with unmodified Trp-111, with Trp-111 oxidized to form oxindolylalanine and with Trp-111 oxidized to form N-formylkynurenine. Oxidation is enhanced by exposure to sunlight. {ECO:0000269\|PubMed:17534989}.
Mass spectrometry:		Mass=2892; Method=Electrospray; Evidence={ECO:0000269\|PubMed:7703226};
Mass spectrometry:		Mass=2982.4; Method=Electrospray; Evidence={ECO:0000269\|PubMed:17534989};
Mass spectrometry:		Mass=2908.4; Method=Electrospray; Note=With oxindolylalanine.; Evidence={ECO:0000269\|PubMed:17534989};
Mass spectrometry:		Mass=2924.4; Method=Electrospray; Note=With N- formylkynurenine.; Evidence={ECO:0000269\|PubMed:17534989};
Pharmaceutical:		The uteroactive properties of Kalata have been discovered by African traditional medicine. It is used as an ingredient of a herbal tea to accelerate childbirth.
Similarity:		Belongs to the cyclotide family. Moebius subfamily. {ECO:0000255\|PROSITE-ProRule:PRU00395}.