|
|
||
|
|
|
|
|
UniProt functional annotation for G8JZS4 | ||
|
|
|
|
|
|
||
| UniProt code: G8JZS4. |
|
||
| Organism: | |
Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482). |
| Taxonomy: | |
Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides. |
|
||
|
||
| Function: | |
Glucoamylase that hydrolyzes alpha-1,4-glucosidic linkages, alpha-1,6-, alpha-1,3- and alpha-1,2-glucosidic linkages during starch degradation. {ECO:0000269|PubMed:18981178, ECO:0000269|PubMed:8955399}. |
|
||
| Catalytic activity: | |
Reaction=Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.; EC=3.2.1.3; Evidence={ECO:0000269|PubMed:18981178}; |
| Cofactor: | |
Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000269|PubMed:18981178}; Note=Binds 1 Ca(2+) ion per subunit. {ECO:0000269|PubMed:18981178}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=1.05 mM for maltose {ECO:0000269|PubMed:18981178}; KM=0.29 mM for maltotriose {ECO:0000269|PubMed:18981178}; KM=0.64 mM for maltotetraose {ECO:0000269|PubMed:18981178}; KM=1.29 mM for maltopentaose {ECO:0000269|PubMed:18981178}; KM=2.71 mM for maltohexaose {ECO:0000269|PubMed:18981178}; KM=4.58 mM for maltoheptaose {ECO:0000269|PubMed:18981178}; KM=6.89 mM for amylose DP17 {ECO:0000269|PubMed:18981178}; KM=0.67 mM for soluble starch {ECO:0000269|PubMed:18981178}; KM=2.39 mM for kojibiose {ECO:0000269|PubMed:18981178}; KM=3.14 mM for nigerose {ECO:0000269|PubMed:18981178}; KM=6.34 mM for isomaltose {ECO:0000269|PubMed:18981178}; KM=0.31 mM for panose {ECO:0000269|PubMed:18981178}; KM=0.16 mM for p-nitrophenyl alpha-glucoside {ECO:0000269|PubMed:18981178}; Note=kcat is 182 sec(-1) for maltose. kcat is 270 sec(-1) for maltotriose. kcat is 321 sec(-1) for maltotetraose. kcat is 434 sec(- 1) for maltopentaose. kcat is 346 sec(-1) for maltohexaose. kcat is 381 sec(-1) for maltoheptaose. kcat is 321 sec(-1) for amylose DP17. kcat is 115 sec(-1) for soluble starch. kcat is 141 sec(-1) for kojibiose. kcat is 207 sec(-1) for nigerose. kcat is 105 sec(-1) for isomaltose. kcat is 160 sec(-1) for panose. kcat is 161 sec(-1) for p-nitrophenyl alpha-glucoside. {ECO:0000269|PubMed:18981178}; pH dependence: Optimum pH is 6.5. {ECO:0000269|PubMed:18981178}; |
| Pathway: | |
Glycan degradation; starch degradation. |
| Subunit: | |
Monomer. {ECO:0000269|PubMed:18981178}. |
| Subcellular location: | |
Periplasm {ECO:0000305|PubMed:8955399}. |
| Similarity: | |
Belongs to the glycosyl hydrolase 97 family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.