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PDBsum entry 2jhc
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Viral protein
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PDB id
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2jhc
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References listed in PDB file
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Key reference
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Title
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Bluetongue virus vp4 is an RNA-Capping assembly line.
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Authors
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G.Sutton,
J.M.Grimes,
D.I.Stuart,
P.Roy.
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Ref.
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Nat Struct Biol, 2007,
14,
449-451.
[DOI no: ]
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PubMed id
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Abstract
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Eukaryotic organisms cap the 5' ends of their messenger RNAs by a series of four
chemical reactions. Some viruses achieve this using a single molecule; the
crystal structure of such an enzyme from bluetongue virus reveals an elongated
modular architecture that provides a scaffold for an assemblage of active sites,
two contributed by a domain of novel structure.
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Figure 1.
(a) Reaction mechanism for a type 1 cap. (b) VP4 domain
organization (domain names defined in text). Poorly ordered
loops are gray. Contact areas between domains are indicated. (c)
VP4 colored by domain, with active sites and ligands colored as
in a; view rotated 120° from b. AdoHcy in N7MTase and
2'OMTase are azure and orange, respectively. Cap analog is
yellow. (d) Active sites mapped onto electrostatic surface
representation of VP4 (colored as in c), and VP4 within BTV core.
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Figure 2.
(a) Left, 2'OMT domain colored from blue (N terminus) to red
(C terminus). Poorly ordered loop is gray. AdoHcy (magenta) and
G5'ppp5'G (blue) substrates are shown. Right, 2'OMTase active
sites of VP4 (purple) and VP39 (cyan). Carbon atoms and bonds of
significant residues are colored gray. VP4 ligands colored as at
left; VP39 AdoHcy and m^7G5'ppp5'G are marine and orange,
respectively. Red and yellow spheres represent cap analog 2'-O
and AdoHcy sulfur, respectively. (b) N7MT domain and AdoHcy, as
in a. Right, stereo view of N7MTase active sites of VP4 and
Ecm1. Carbon atoms and bonds of VP4 are gray, with guanine bases
purple and AdoHcy pink. Ecm1 is in olive, with cap analog orange
(blue sphere, N7) and AdoHcy lime (yellow spheres, sulfurs).
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The above figures are
reprinted
by permission from Macmillan Publishers Ltd:
Nat Struct Biol
(2007,
14,
449-451)
copyright 2007.
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