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UniProt functional annotation for Q9XZT6 | ||
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| UniProt code: Q9XZT6. |
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| Organism: | |
Drosophila melanogaster (Fruit fly). |
| Taxonomy: | |
Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora. |
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| Function: | |
Deoxyribonucleoside kinase that has a broad specificity phosphorylating thymidine, 2'-deoxyriboadenosine, 2'-deoxyribocytidine and 2'-deoxyriboguanosine. Specificity is higher for pyrimidine nucleosides. Several anti-viral and anti-cancer nucleoside analogs are also efficiently phosphorylated. {ECO:0000269|PubMed:10446143, ECO:0000269|PubMed:10692477, ECO:0000269|PubMed:16008571}. |
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| Catalytic activity: | |
Reaction=a 2'-deoxyribonucleoside + ATP = a 2'-deoxyribonucleoside 5'- phosphate + ADP + H(+); Xref=Rhea:RHEA:12140, ChEBI:CHEBI:15378, ChEBI:CHEBI:18274, ChEBI:CHEBI:30616, ChEBI:CHEBI:65317, ChEBI:CHEBI:456216; EC=2.7.1.145; Evidence={ECO:0000269|PubMed:10446143, ECO:0000269|PubMed:10692477, ECO:0000269|PubMed:16008571, ECO:0000269|PubMed:9461577}; |
| Activity regulation: | |
Subject to feedback inhibition by dTTP. {ECO:0000269|PubMed:16008571}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=0.9 uM for thymidine {ECO:0000269|PubMed:10446143, ECO:0000269|PubMed:10692477}; KM=1 uM for deoxycytidine {ECO:0000269|PubMed:10446143, ECO:0000269|PubMed:10692477}; KM=109 uM for deoxyadenosine {ECO:0000269|PubMed:10446143, ECO:0000269|PubMed:10692477}; KM=654 uM for deoxyguanosine {ECO:0000269|PubMed:10446143, ECO:0000269|PubMed:10692477}; Vmax=29.4 mmol/min/mg enzyme with thymidine as substrate {ECO:0000269|PubMed:10446143, ECO:0000269|PubMed:10692477}; Vmax=28.7 mmol/min/mg enzyme with deoxycytidine as substrate {ECO:0000269|PubMed:10446143, ECO:0000269|PubMed:10692477}; Vmax=35.5 mmol/min/mg enzyme with deoxyadenosine as substrate {ECO:0000269|PubMed:10446143, ECO:0000269|PubMed:10692477}; Vmax=37.7 mmol/min/mg enzyme with deoxyguanosine as substrate {ECO:0000269|PubMed:10446143, ECO:0000269|PubMed:10692477}; |
| Subunit: | |
Monomer. {ECO:0000269|PubMed:9461577}. |
| Similarity: | |
Belongs to the DCK/DGK family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.