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PDBsum entry 2j5h
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Hormone/growth factor
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PDB id
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2j5h
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References listed in PDB file
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Key reference
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Title
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Solution structure of mouse cripto cfc domain and its inactive variant trp107ala.
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Authors
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L.Calvanese,
A.Saporito,
D.Marasco,
G.D'Auria,
G.Minchiotti,
C.Pedone,
L.Paolillo,
L.Falcigno,
M.Ruvo.
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Ref.
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J Med Chem, 2006,
49,
7054-7062.
[DOI no: ]
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PubMed id
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Abstract
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We report here for the first time the solution structures at pH 3 and pH 6 of
the synthetic CFC domain of mouse Cripto and of the point mutated variant W107A
that is unable to bind to the Alk4 Cripto receptor. NMR data confirm that the
CFC domain has a C1-C4, C2-C6, C3-C5 disulfide pattern and show that structures
are rather flexible and globally extended, with three noncanonical antiparallel
strands. His104 and Trp107 side chains protrude from a protein edge and are
strongly exposed to solvent, supporting previous evidence of direct involvement
in receptor binding. On the opposite molecule side, several nonpolar residues
are gathered, forming a large hydrophobic patch that supposedly acts as
interface with the cell membrane or the adjacent EGF-like domain. A second
hydrophilic patch surrounding His104 and Trp107 is present only in the wild type
variant, suggesting a possible involvement in modulating Alk4 recognition.
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