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PDBsum entry 2j1v
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Carbohydrate-binding protein
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PDB id
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2j1v
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References listed in PDB file
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Key reference
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Title
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Blood group antigen recognition by a streptococcus pneumoniae virulence factor.
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Authors
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A.B.Boraston,
D.Wang,
R.D.Burke.
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Ref.
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J Biol Chem, 2006,
281,
35263-35271.
[DOI no: ]
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PubMed id
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Abstract
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The Streptococcus pneumoniae fucose utilization operon includes a gene encoding
a virulence factor that belongs to family 98 in the glycoside hydrolase
classification. This protein contains a C-terminal triplet of fucose binding
modules that have significant amino acid sequence identity with the Anguilla
anguilla fucolectin. Functional studies of these fucose binding modules reveal
binding to fucosylated oligosaccharides and suggest the importance of
multivalent binding. The high resolution crystal structures of ligand bound
forms of one fucose binding module uncovers the molecular basis of fucose, ABH
blood group antigen, and Lewisy antigen binding. These studies are extended by
fluorescence microscopy to show specific binding to mouse lung tissue. These
modules define a new family of carbohydrate binding modules now classified as
family 47.
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Figure 5.
FIGURE 5. Binding site architectures and electron density
of fucose (A), the type II H-trisaccharide (B), the blood group
A-tetrasaccharide analogue (C), and the Lewis^y antigen (D)
bound to SpX-1. Electron density maps are
maximum-likelihood(17)/  [A] (27) weighted
2F[obs] - F[calc] electron density maps contoured at 1 (0.39,
0.48, 0.23, and 0.28 electrons/Å^3) for the fucose, type
II blood group H-trisaccharide, blood group A-tetrasaccharide
analogue, and Lewis^y antigen complexes, respectively. Relevant
residues involved in binding are shown in gray stick
representation and labeled.
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Figure 6.
FIGURE 6. Hydrogen bonding schematics of fucose (A), the
type II H-trisaccharide (B), the blood group A-tetrasaccharide
analogue (C), and the Lewis^y antigen (D) bound to SpX-1.
Potential hydrogen bonds are shown as dotted lines. Waters are
shown as gray spheres. Potential hydrogen bond distances were
between 2.5 and 3.3 Å.
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The above figures are
reprinted
by permission from the ASBMB:
J Biol Chem
(2006,
281,
35263-35271)
copyright 2006.
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