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UniProt functional annotation for P16521 | ||
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| UniProt code: P16521. |
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| Organism: | |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). |
| Taxonomy: | |
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. |
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| Function: | |
Ribosome-dependent ATPase that functions in cytoplasmic translation elongation (PubMed:7657623, PubMed:6456269, PubMed:29300771). Required for the ATP-dependent release of deacylated tRNA from the ribosomal E-site during protein biosynthesis (PubMed:7657623). Stimulates the eEF1A-dependent binding of aminoacyl- tRNA to the ribosomal A-site, which has reduced affinity for tRNA as long as the E-site is occupied (PubMed:7657623). Plays a role as a negative regulator of the GCN2 kinase activity; impairs GCN1-mediated GCN2 activation on ribosomes by reducing GCN1-ribosome affinity, and hence GCN2-mediated eIF-2-alpha phosphorylation in amino acid-starved or repleted cells (PubMed:22888004). {ECO:0000269|PubMed:22888004, ECO:0000269|PubMed:29300771, ECO:0000269|PubMed:6456269, ECO:0000269|PubMed:7657623}. |
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| Catalytic activity: | |
Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; Evidence={ECO:0000269|PubMed:29300771, ECO:0000269|PubMed:6456269, ECO:0000269|PubMed:7657623, ECO:0000269|PubMed:7957240}; |
| Activity regulation: | |
Inhibited by the translational inhibitors neomycin and alpha-sarcin, which suppress the ATPase activity. {ECO:0000269|PubMed:7957240}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=0.126 mM for ATP {ECO:0000269|PubMed:7957240}; KM=0.125 mM for GTP {ECO:0000269|PubMed:7957240}; Vmax=15.2 umol/min/mg enzyme with ATP as substrate {ECO:0000269|PubMed:7957240}; |
| Pathway: | |
Protein biosynthesis; polypeptide chain elongation. {ECO:0000305}. |
| Subunit: | |
Monomer. Interacts with elongation factor 1A (eEF1A). Interacts through its N-terminus with 18S rRNA. Associates with ribosomes (PubMed:22888004). {ECO:0000269|PubMed:12493761, ECO:0000269|PubMed:22888004, ECO:0000269|PubMed:9553076, ECO:0000269|PubMed:9990316}. |
| Subcellular location: | |
Cytoplasm {ECO:0000269|PubMed:14562095}. |
| Induction: | |
Increases in strong (1 mM) hydrogen peroxide stress, and decreases in milder (0.5 mM) hydrogen peroxide stress. {ECO:0000269|PubMed:33260587}. |
| Domain: | |
The heat repeats and the C-terminal domain are necessary for impairing GCN1 function on ribosomes, and hence preventing GCN2 kinase activity in amino acid-starved or repleted cells (PubMed:22888004). {ECO:0000269|PubMed:22888004}. |
| Miscellaneous: | |
Present with 870578 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}. |
| Similarity: | |
Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.