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PDBsum entry 2is9
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Transcription
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PDB id
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2is9
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References listed in PDB file
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Key reference
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Title
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Structural basis for the function of dcn-1 in protein neddylation.
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Authors
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X.Yang,
J.Zhou,
L.Sun,
Z.Wei,
J.Gao,
W.Gong,
R.M.Xu,
Z.Rao,
Y.Liu.
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Ref.
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J Biol Chem, 2007,
282,
24490-24494.
[DOI no: ]
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PubMed id
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Abstract
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Covalent modification by Nedd8 (neddylation) stimulates the ubiquitin-protein
isopeptide ligase (E3) activities of Cullins. DCN-1, an evolutionarily conserved
protein, promotes neddylation of Cullins in vivo, binds directly to Nedd8, and
associates with Cdc53 in the budding yeast Saccharomyces cerevisiae. The 1.9A
resolution structure of yeast DCN-1 shows that the region encompassing residues
66-269 has a rectangular parallelepiped-like all alpha-helical structures,
consisting of an EF-hand motif N-terminal domain and a closely juxtaposed
C-terminal domain with six alpha-helices. The EF-hand motif structure is highly
similar to that of the c-Cbl ubiquitin E3 ligase. We also demonstrate that DCN-1
directly binds to Rbx-1, a factor important for protein neddylation. The
structural and biochemical results are consistent with the role of DCN-1 as a
scaffold protein in a multisubunit neddylation E3 ligase complex.
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Figure 2.
Structure of DCN-1.A, a stereo ribbon diagram showing the
overall structure of DCN-1. The structure is colored from the N
to the C termini with blue to red. B, a surface representation
of the DCN-1 structure. B1 (side 1) and B3 (side 2) show the
distribution of conserved residues on the two sides of the
protein surface. Absolutely conserved residues are indicated in
blue, and highly conserved residues are shown in green, using
the convention defined in the legend for Fig. 1. The arrow
points to the position of Glu-260, which is colored in red.
Electrostatic potential on the protein surface is shown in B2
and B4, which are oriented the same as in B1 and B3,
respectively. Positively charged regions are shown in red,
negatively charged regions are in blue, and neutral ones are in
white.
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Figure 3.
Superposition of c-Cbl and DCN-1.A, a stereo diagram of
superimposed DCN-1 (blue) and c-Cbl (gray) EF-hand motifs. A red
sphere represents a calcium ion bound to the c-Cbl EF-hand
motif. B, superposition of c-Cbl (only EF-hand and helix bundle
motifs are shown) with DCN-1 to show the different spatial
arrangement of the two helix bundle motifs.
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The above figures are
reprinted
by permission from the ASBMB:
J Biol Chem
(2007,
282,
24490-24494)
copyright 2007.
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