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UniProt functional annotation for Q91WR5 | ||
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| UniProt code: Q91WR5. |
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| Organism: | |
Mus musculus (Mouse). |
| Taxonomy: | |
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. |
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| Function: | |
NADP-dependent 17-alpha-hydroxysteroid dehydrogenase that converts 5-alpha-androstane-3,17-dione into androsterone. Has lower 3- alpha-hydroxysteroid dehydrogenase activity. Has broad substrate specificity and acts on various 17-alpha-hydroxysteroids, 17- ketosteroids, 3-alpha hydroxysteroids and 3-ketosteroids. Reduction of keto groups is strictly stereoselective. Reduction of 17-ketosteroids yields only 17-alpha-hydroxysteroids. Likewise, reduction of 3- ketosteroids yields only 3-alpha-hydroxysteroids. {ECO:0000269|PubMed:15577209, ECO:0000269|PubMed:16018803, ECO:0000269|PubMed:17034817, ECO:0000269|PubMed:17442338, ECO:0000269|PubMed:20124700}. |
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| Catalytic activity: | |
Reaction=3alpha-hydroxy-5alpha-androstan-17-one + NADP(+) = 5alpha- androstan-3,17-dione + H(+) + NADPH; Xref=Rhea:RHEA:20377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15994, ChEBI:CHEBI:16032, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.1.1.209; Evidence={ECO:0000269|PubMed:15577209, ECO:0000269|PubMed:17442338, ECO:0000269|PubMed:20124700}; |
| Catalytic activity: | |
Reaction=3alpha-hydroxy-5alpha-androstan-17-one + NAD(+) = 5alpha- androstan-3,17-dione + H(+) + NADH; Xref=Rhea:RHEA:20381, ChEBI:CHEBI:15378, ChEBI:CHEBI:15994, ChEBI:CHEBI:16032, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.209; Evidence={ECO:0000269|PubMed:15577209, ECO:0000269|PubMed:17442338, ECO:0000269|PubMed:20124700}; |
| Activity regulation: | |
Inhibited by high concentrations of substrate. {ECO:0000269|PubMed:15577209}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=1.8 uM for NADP {ECO:0000269|PubMed:15577209, ECO:0000269|PubMed:17442338}; KM=19 uM for androstenedione {ECO:0000269|PubMed:15577209, ECO:0000269|PubMed:17442338}; KM=0.5 uM for 5-beta-pregnane-3-alpha,20-alpha-diol {ECO:0000269|PubMed:15577209, ECO:0000269|PubMed:17442338}; KM=0.5 uM for 5-beta-pregnane-3,20-dione {ECO:0000269|PubMed:15577209, ECO:0000269|PubMed:17442338}; KM=0.6 uM for epitestosterone {ECO:0000269|PubMed:15577209, ECO:0000269|PubMed:17442338}; KM=0.3 uM for androst-4-ene-3,17-dione {ECO:0000269|PubMed:15577209, ECO:0000269|PubMed:17442338}; pH dependence: Optimum pH is 10.0. {ECO:0000269|PubMed:15577209, ECO:0000269|PubMed:17442338}; |
| Subunit: | |
Monomer. {ECO:0000269|PubMed:15577209, ECO:0000269|PubMed:17034817, ECO:0000269|PubMed:17442338, ECO:0000269|PubMed:17909281, ECO:0000269|PubMed:19237748}. |
| Subcellular location: | |
Cytoplasm {ECO:0000250}. |
| Tissue specificity: | |
Detected in kidney and brain. {ECO:0000269|PubMed:15577209, ECO:0000269|PubMed:16018803}. |
| Similarity: | |
Belongs to the aldo/keto reductase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.