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UniProt functional annotation for Q6N5P6 | ||
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| UniProt code: Q6N5P6. |
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| Organism: | |
Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009). |
| Taxonomy: | |
Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Bradyrhizobiaceae; Rhodopseudomonas. |
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| Function: | |
Catalyzes the attachment of proline to tRNA(Pro) in a two- step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). Can inadvertently accommodate and process cysteine. The misacylated Cys-tRNA(Pro) is not edited by ProRS. |
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| Catalytic activity: | |
Reaction=ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl- tRNA(Pro); Xref=Rhea:RHEA:14305, Rhea:RHEA-COMP:9700, Rhea:RHEA- COMP:9702, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:60039, ChEBI:CHEBI:78442, ChEBI:CHEBI:78532, ChEBI:CHEBI:456215; EC=6.1.1.15; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=0.28 mM for proline {ECO:0000269|PubMed:12130657}; KM=0.17 mM for cysteine {ECO:0000269|PubMed:12130657}; |
| Subunit: | |
Homodimer. {ECO:0000250}. |
| Subcellular location: | |
Cytoplasm. |
| Similarity: | |
Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.