PDBsum entry 2hsf

Transcription regulation

PDB id: 2hsf

Contents

Protein chain

92 a.a.

References listed in PDB file

Key reference

• Title: Solution structure of the DNA-Binding domain of the heat shock transcription factor determined by multidimensional heteronuclear magnetic resonance spectroscopy.
• Authors: F.F.Damberger, J.G.Pelton, C.J.Harrison, H.C.Nelson, D.E.Wemmer.
• Ref.: Protein Sci, 1994, 3, 1806-1821. [DOI no: 10.1002/pro.5560031020]
• PubMed id: 7849597

Note In the PDB file this reference is annotated as "TO BE PUBLISHED". The citation details given above were identified by an automated search of PubMed on title and author names, giving a percentage match of 96%.

Abstract

The solution structure of the 92-residue DNA-binding domain of the heat shock transcription factor from Kluyveromyces lactis has been determined using multidimensional NMR methods. Three-dimensional (3D) triple resonance, 1H-13C-13C-1H total correlation spectroscopy, and 15N-separated total correlation spectroscopy-heteronuclear multiple quantum correlation experiments were used along with various 2D spectra to make nearly complete assignments for the backbone and side-chain 1H, 15N, and 13C resonances. Five-hundred eighty-three NOE constraints identified in 3D 13C- and 15N-separated NOE spectroscopy (NOESY)-heteronuclear multiple quantum correlation spectra and a 4-dimensional 13C/13C-edited NOESY spectrum, along with 35 phi, 9 chi 1, and 30 hydrogen bond constraints, were used to calculate 30 structures by hybrid distance geometry/stimulated annealing protocol, of which 24 were used for structural comparison. The calculations revealed that a 3-helix bundle packs against a small 4-stranded antiparallel beta-sheet. The backbone RMS deviation (RMSD) for the family of structures was 1.03 +/- 0.19 A with respect to the average structure. The topology is analogous to that of the C-terminal domain of the catabolite gene activator protein and appears to be in the helix-turn-helix family of DNA-binding proteins. The overall fold determined by the NMR data is consistent with recent crystallographic work on this domain (Harrison CJ, Bohm AA, Nelson HCM, 1994, Science 263:224) as evidenced by RMSD between backbone atoms in the NMR and X-ray structures of 1.77 +/- 0.20 A. Several differences were identified some of which may be due to protein-protein interactions in the crystal.

Secondary reference #1

• Title: Crystal structure of the DNA binding domain of the heat shock transcription factor.
• Authors: C.J.Harrison, A.A.Bohm, H.C.Nelson.
• Ref.: Science, 1994, 263, 224-227. [DOI no: 10.1126/science.8284672]
• PubMed id: 8284672