PDBsum entry 2hg9

Photosynthesis/membrane protein

PDB id: 2hg9

Contents

Protein chains

281 a.a.

302 a.a.

241 a.a.

Ligands

PO4 ×4

BCL ×4

BPH ×2

U10 ×2

CDL

PCK

LDA ×7

GOL ×6

PC7

Metals

_CL ×2

_FE

__K

Waters ×441

References listed in PDB file

Key reference

• Title: Brominated lipids identify lipid binding sites on the surface of the reaction center from rhodobacter sphaeroides.
• Authors: A.W.Roszak, A.T.Gardiner, N.W.Isaacs, R.J.Cogdell.
• Ref.: Biochemistry, 2007, 46, 2909-2916. [DOI no: 10.1021/bi062154i]
• PubMed id: 17315985

Abstract

This study describes the use of brominated phospholipids to distinguish between lipid and detergent binding sites on the surface of a typical alpha-helical membrane protein. Reaction centers isolated from Rhodobacter sphaeroides were cocrystallized with added brominated phospholipids. X-ray structural analysis of these crystals has revealed the presence of two lipid binding sites from the characteristic strong X-ray scattering from the bromine atoms. These results demonstrate the usefulness of this approach to mapping lipid binding sites at the surface of membrane proteins.