PDBsum entry 2gzi

Antibiotic/antibiotic inhibitor

PDB id: 2gzi

Contents

Protein chains

83 a.a. *

132 a.a. *

Ligands

PO4

Metals

_ZN

Waters ×201

* Residue conservation analysis

PDB id:

2gzi

Name:

Antibiotic/antibiotic inhibitor

Title:

Crystal structure of the e9 dnase domain with a mutant immunity protein im9 (v34a)

Structure:

Colicin-e9 immunity protein. Chain: a. Synonym: imme9, microcin-e9 immunity protein. Engineered: yes. Mutation: yes. Colicin-e9. Chain: b. Fragment: colicin e9,c-terminal domain,dnase domain. Engineered: yes

Source:

Escherichia coli k12. Organism_taxid: 83333. Strain: k-12. Gene: imm, ceie9. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Gene: col, cei. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.70Å R-factor: 0.216 R-free: 0.256

Authors:

P.S.Santi,O.O.Kolade,U.C.Kuhlmann,C.Kleanthous,A.M.Hemmings

Key ref:

U.C.Kuhlmann et al. Crystal structure of the complex of the colicin e9 dnase domain with a mutant immunity protein, Im9 (V34a). To be published, .

Date:

11-May-06 Release date: 18-Mar-08

Protein chain

P13479  (IMM9_ECOLX) -  Colicin-E9 immunity protein from Escherichia coli

Seq: 86 a.a.

Struc: 83 a.a.*

Protein chain

P09883  (CEA9_ECOLX) -  Colicin-E9 from Escherichia coli

Seq: 582 a.a.

Struc: 132 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chain B: E.C.3.1.-.-