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UniProt functional annotation for O23736 | ||
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| UniProt code: O23736. |
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| Organism: | |
Brassica juncea (Indian mustard) (Sinapis juncea). |
| Taxonomy: | |
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica. |
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| Function: | |
Participates in the detoxification process. {ECO:0000269|PubMed:9119067, ECO:0000269|PubMed:9620267}. |
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| Catalytic activity: | |
Reaction=ATP + L-cysteine + L-glutamate = ADP + H(+) + L-gamma- glutamyl-L-cysteine + phosphate; Xref=Rhea:RHEA:13285, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616, ChEBI:CHEBI:35235, ChEBI:CHEBI:43474, ChEBI:CHEBI:58173, ChEBI:CHEBI:456216; EC=6.3.2.2; |
| Pathway: | |
Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2. |
| Subunit: | |
Homodimer or monomer when oxidized or reduced, respectively. {ECO:0000269|PubMed:16766527}. |
| Subcellular location: | |
Plastid, chloroplast {ECO:0000250}. |
| Induction: | |
Up-regulated by cadmium and cu(2+). {ECO:0000269|PubMed:9119067, ECO:0000269|PubMed:9620267}. |
| Ptm: | |
The Cys-178-Cys-398 disulfide bridge is known to modulate the enzyme activity according to the redox status. The oxidized form constitutes the active enzyme. |
| Similarity: | |
Belongs to the carboxylate-amine ligase family. Glutamate-- cysteine ligase type 2 subfamily. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.