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PDBsum entry 2gv5
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References listed in PDB file
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Key reference
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Title
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Structural role of sfi1p-Centrin filaments in budding yeast spindle pole body duplication.
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Authors
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S.Li,
A.M.Sandercock,
P.Conduit,
C.V.Robinson,
R.L.Williams,
J.V.Kilmartin.
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Ref.
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J Cell Biol, 2006,
173,
867-877.
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PubMed id
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Abstract
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Centrins are calmodulin-like proteins present in centrosomes and yeast spindle
pole bodies (SPBs) and have essential functions in their duplication. The
Saccharomyces cerevisiae centrin, Cdc31p, binds Sfi1p on multiple conserved
repeats; both proteins localize to the SPB half-bridge, where the new SPB is
assembled. The crystal structures of Sfi1p-centrin complexes containing several
repeats show Sfi1p as an alpha helix with centrins wrapped around each repeat
and similar centrin-centrin contacts between each repeat. Electron microscopy
(EM) shadowing of an Sfi1p-centrin complex with 15 Sfi1 repeats and 15 centrins
bound showed filaments 60 nm long, compatible with all the Sfi1 repeats as a
continuous alpha helix. Immuno-EM localization of the Sfi1p N and C termini
showed Sfi1p-centrin filaments spanning the length of the half-bridge with the
Sfi1p N terminus at the SPB. This suggests a model for SPB duplication where the
half-bridge doubles in length by association of the Sfi1p C termini, thereby
providing a new Sfi1p N terminus to initiate SPB assembly.
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