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PDBsum entry 2grm
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Transcription
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PDB id
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2grm
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References listed in PDB file
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Key reference
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Title
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Molecular basis for control of conjugation by bacterial pheromone and inhibitor peptides.
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Authors
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B.K.Kozlowicz,
K.Shi,
Z.Y.Gu,
D.H.Ohlendorf,
C.A.Earhart,
G.M.Dunny.
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Ref.
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Mol Microbiol, 2006,
62,
958-969.
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PubMed id
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Abstract
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In many bacteria expression of lateral gene transfer and of virulence factors is
controlled by cell-cell signalling systems. Molecular interactions of microbial
signal molecules with their cognate receptors are not well understood. For the
Enterococcus faecalis conjugative plasmid pCF10, the PrgX protein serves as a
molecular switch controlling expression of conjugation and virulence genes
encoded by the plasmid. The induction state of a pCF10-carrying donor cell is
determined by the ratio of two signalling peptides, cCF10 pheromone and iCF10
inhibitor. Recent analysis of PrgX/cCF10 interactions suggests a mechanism for
conversion to the induced state. However, the means by which iCF10 peptide
antagonizes cCF10 activity is unclear, and it has been suggested that inhibitor
peptides block import of pheromone peptides. We now show that both of these
peptides interact with the same binding pocket of PrgX, but they differentially
alter the conformation of the protein and its oligomerization state, resulting
in opposing biological activities.
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