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UniProt functional annotation for P39135 | ||
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| UniProt code: P39135. |
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| Organism: | |
Bacillus subtilis (strain 168). |
| Taxonomy: | |
Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus. |
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| Function: | |
Activates the seven peptidyl carrier protein (PCP) domains of the first three subunits (SrfAA, SrfAB and SrfAC) of surfactin synthetase by transferring the 4'-phosphopantetheinyl moiety of coenzyme A (CoA) to a serine residue. Required for cells of B.subtilis to become producers of the lipopeptide antibiotics surfactin and plipastatin B1. {ECO:0000269|PubMed:8939709, ECO:0000269|PubMed:9484229}. |
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| Catalytic activity: | |
Reaction=apo-[ACP] + CoA = adenosine 3',5'-bisphosphate + H(+) + holo- [ACP]; Xref=Rhea:RHEA:12068, Rhea:RHEA-COMP:9685, Rhea:RHEA- COMP:9690, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:57287, ChEBI:CHEBI:58343, ChEBI:CHEBI:64479; EC=2.7.8.7; Evidence={ECO:0000269|PubMed:11451672, ECO:0000269|PubMed:9484229}; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=0.7 uM for coenzyme A {ECO:0000269|PubMed:9484229}; KM=1.3 uM for a peptide fragment encompassing residues 960-1084 of surfactin synthetase SrfAB subunit {ECO:0000269|PubMed:9484229}; KM=1.8 uM for a peptide fragment encompassing residues 2006-2123 of surfactin synthetase SrfAB subunit {ECO:0000269|PubMed:9484229}; KM=3.7 uM for Streptomyces mobaraensis apo-PCP BlmI {ECO:0000269|PubMed:11451672}; KM=11 uM for Streptomyces glaucescens apo-ACP TcmM {ECO:0000269|PubMed:11451672}; Note=kcat is 104 min(-1) with a peptide fragment encompassing residues 960-1084 of surfactin synthetase SrfAB subunit as substrate. kcat is 56 min(-1) with a peptide fragment encompassing residues 2006-2123 of surfactin synthetase SrfAB subunit as substrate (PubMed:9484229). kcat is 7.2 min(-1) with S.mobaraensis apo-PCP BlmI as substrate and 0.89 min(-1) with S.glaucescens apo-ACP TcmM as substrate (PubMed:11451672). {ECO:0000269|PubMed:11451672, ECO:0000269|PubMed:9484229}; pH dependence: Optimum pH is 6.0. Displays less than 20% of Vmax at both pH 5.0 and pH 7.0. {ECO:0000269|PubMed:9484229}; |
| Subunit: | |
Monomer in solution. |
| Similarity: | |
Belongs to the P-Pant transferase superfamily. Gsp/Sfp/HetI/AcpT family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.