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PDBsum entry 2g7c
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References listed in PDB file
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Key reference
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Title
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Carbohydrate recognition by clostridium difficile toxin a.
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Authors
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A.Greco,
J.G.Ho,
S.J.Lin,
M.M.Palcic,
M.Rupnik,
K.K.Ng.
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Ref.
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Nat Struct Mol Biol, 2006,
13,
460-461.
[DOI no: ]
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PubMed id
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Abstract
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Clostridium difficile TcdA is a large toxin that binds carbohydrates on
intestinal epithelial cells. A 2-A resolution cocrystal structure reveals two
molecules of alpha-Gal-(1,3)-beta-Gal-(1,4)-beta-GlcNAcO(CH(2))(8)CO(2)CH(3)
binding in an extended conformation to TcdA. Residues forming key contacts with
the trisaccharides are conserved in all seven putative binding sites in TcdA,
suggesting a mode of multivalent binding that may be exploited for the rational
design of novel therapeutics.
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Figure 1.
Figure 1. Structure of TcdA-f2. (a) Ribbon structure and
schematic with SRs (dark blue), SR3 and SR6 (cyan), and LRs
(green) highlighted. (b) Ribbon representation of TcdA-f2 and
bound trisaccharides, viewed from the N terminus. The
reducing-end  -GlcNAc
is colored yellow.
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Figure 2.
Figure 2. TcdA-f2 bound to CD-grease at BS1A. Hydrogen bonds
are drawn as dashed lines.
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The above figures are
reprinted
by permission from Macmillan Publishers Ltd:
Nat Struct Mol Biol
(2006,
13,
460-461)
copyright 2006.
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