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UniProt functional annotation for P76341 | ||
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| UniProt code: P76341. |
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| Organism: | |
Escherichia coli (strain K12). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
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| Function: | |
Catalyzes the hydrolysis of 5-hydroxyisourate (HIU) to 2-oxo- 4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU). {ECO:0000269|PubMed:16098976}. |
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| Catalytic activity: | |
Reaction=5-hydroxyisourate + H2O = 5-hydroxy-2-oxo-4-ureido-2,5- dihydro-1H-imidazole-5-carboxylate + H(+); Xref=Rhea:RHEA:23736, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:18072, ChEBI:CHEBI:58639; EC=3.5.2.17; Evidence={ECO:0000269|PubMed:16098976}; |
| Subunit: | |
Homotetramer. {ECO:0000269|PubMed:12542701, ECO:0000269|PubMed:16723258}. |
| Subcellular location: | |
Periplasm {ECO:0000269|Ref.3}. |
| Induction: | |
Induced by H(2)O(2), via the HprR/HprS two-component system, and by Cu(2+), via the CusS/CusR two-component system. HprR and CusR bind to essentially the same sequence within the hiuH promoter region. {ECO:0000269|PubMed:25568260, ECO:0000269|PubMed:27983483}. |
| Mass spectrometry: | |
Mass=13013; Method=Electrospray; Evidence={ECO:0000269|PubMed:12542701}; |
| Miscellaneous: | |
Despite its homology to transthyretins, is not able to bind to the thyroid hormones thyroxine (T4) and triiodothyronine (T3). |
| Miscellaneous: | |
Although some crystallographic structures were found to contain zinc, the purified protein does not contain bound metal ions, and does not require zinc or other metal ions for enzyme activity. |
| Miscellaneous: | |
HIU hydrolysis also occurs spontaneously, but more slowly. |
| Similarity: | |
Belongs to the transthyretin family. 5-hydroxyisourate hydrolase subfamily. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.