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UniProt functional annotation for Q8U4Q3 | ||
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| UniProt code: Q8U4Q3. |
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| Organism: | |
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1). |
| Taxonomy: | |
Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae; Pyrococcus. |
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| Function: | |
Hydrolyzes RNA 2',3'-cyclic phosphodiester to an RNA 2'- phosphomonoester. In vitro, ligates 5' and 3' half-tRNA molecules with 2',3'-cyclic phosphate and 5'-hydroxyl termini, respectively, to the product containing the 2'-5' phosphodiester linkage. Ligase activity requires GTP, but GTP hydrolysis is not required for the reaction, which is reversible. Ligase activity is weak compared to the phosphodiesterase activity. {ECO:0000269|PubMed:19155324}. |
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| Catalytic activity: | |
Reaction=H2O + RNA(n)-2',3'-cyclic phosphate = H(+) + RNA(n)- 2'- phosphate; Xref=Rhea:RHEA:11828, Rhea:RHEA-COMP:13350, Rhea:RHEA- COMP:13351, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:136820, ChEBI:CHEBI:136821; EC=3.1.4.58; Evidence={ECO:0000255|HAMAP- Rule:MF_01940, ECO:0000269|PubMed:19155324}; |
| Similarity: | |
Belongs to the 2H phosphoesterase superfamily. ThpR family. {ECO:0000255|HAMAP-Rule:MF_01940}. |
Annotations taken from UniProtKB at the EBI.