UniProt functional annotation for P15047



	UniProt functional annotation for P15047

UniProt code: P15047.

Organism: Escherichia coli (strain K12).

Taxonomy: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia.

Function: Involved in the biosynthesis of the siderophore enterobactin (enterochelin), which is a macrocyclic trimeric lactone of N-(2,3- dihydroxybenzoyl)-serine. Catalyzes the reversible NAD-dependent oxidation of the C3-hydroxyl group of 2,3-dihydro-2,3-dihydroxybenzoate (2,3-diDHB), producing the transient intermediate 2-hydroxy-3-oxo-4,6- cyclohexadiene-1-carboxylate, which undergoes rapid aromatization to the final product, 2,3-dihydroxybenzoate (2,3-DHB). Only the compounds with a C3-hydroxyl group such as methyl 2,3-dihydro-2,3- dihydroxybenzoate, methyl-3-hydroxy-1,4-cyclohexadiene-1-carboxylate, trans-3-hydroxy-2-cyclohexene-1-carboxylate, cis-3-hydroxy-4- cyclohexene-1-carboxylate, cis-3-hydroxycyclohexane-1-carboxylic acid are oxidized to the corresponding ketone products. The stereospecificity of the C3 allylic alcohol group oxidation is 3R in a 1R,3R dihydro substrate. It can also increase the DHB-AMP ligase activity of EntE by interaction EntE. {ECO:0000269|PubMed:21166461, ECO:0000269|PubMed:2144454, ECO:0000269|PubMed:2521622}.

Catalytic activity: Reaction=(2S,3S)-2,3-dihydroxy-2,3-dihydrobenzoate + NAD(+) = 2,3- dihydroxybenzoate + H(+) + NADH; Xref=Rhea:RHEA:23824, ChEBI:CHEBI:15378, ChEBI:CHEBI:36654, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:58764; EC=1.3.1.28; Evidence={ECO:0000269|PubMed:2144454};

Activity regulation: Inhibited by cis-2-hydroxy-3-cyclohexen-1- carboxylate, cis-2-hydroxycyclohexane-1-carboxylate and trans-2- hydroxycyclohexane-1-carboxylate. {ECO:0000269|PubMed:2144454}.

Biophysicochemical properties: Kinetic parameters: KM=0.26 mM for methyl 2,3-dihydro-2,3-dihydroxybenzoate (at pH 7.4 and 37 degrees Celsius) {ECO:0000269|PubMed:2144454}; KM=0.3 mM for 2,3-dihydro-2,3-dihydroxybenzoate (at pH 7.4 and 37 degrees Celsius) {ECO:0000269|PubMed:2144454}; KM=1.7 mM for methyl-3-hydroxy-1,4-cyclohexadiene-1-carboxylate (at pH 7.4 and 37 degrees Celsius) {ECO:0000269|PubMed:2144454}; KM=1.9 mM for trans-3-hydroxy-2-cyclohexene-1-carboxylate (at pH 7.4 and 37 degrees Celsius) {ECO:0000269|PubMed:2144454}; KM=2.8 mM for cis-3-hydroxy-4-cyclohexene-1-carboxylate (at pH 7.4 and 37 degrees Celsius) {ECO:0000269|PubMed:2144454}; KM=4.1 mM for cis-3-hydroxycyclohexane-1-carboxylic acid (at pH 7.4 and 37 degrees Celsius) {ECO:0000269|PubMed:2144454}; KM=16.5 mM for trans-3,5-cyclohexadiene-1,2-diol (at pH 7.4 and 37 degrees Celsius) {ECO:0000269|PubMed:2144454}; KM=25.2 mM for trans-3-hydroxycyclohexane-1-carboxylic acid (at pH 7.4 and 37 degrees Celsius) {ECO:0000269|PubMed:2144454}; KM=83.3 mM for 2-cyclohexen-1-ol (at pH 7.4 and 37 degrees Celsius) {ECO:0000269|PubMed:2144454}; KM=168 mM for cis-3,5-cyclohexadiene-1,2-diol (at pH 7.4 and 37 degrees Celsius) {ECO:0000269|PubMed:2144454}; Note=Kcat is 5550 min(-1) for dehydrogenase activity with 2,3- dihydro-2,3-dihydroxybenzoate as substrate (at pH 7.4 and 37 degrees Celsius). Kcat is 1380 min(-1) for dehydrogenase activity with cis-3- hydroxy-4-cyclohexene-1-carboxylate as substrate (at pH 7.4 and 37 degrees Celsius). Kcat is 1050 min(-1) for dehydrogenase activity with 2,3-dihydro-2,3-dihydroxybenzoate as substrate (at pH 7.4 and 37 degrees Celsius). Kcat is 1000 min(-1) for dehydrogenase activity with cis-3,5-cyclohexadiene-1,2-diol as substrate (at pH 7.4 and 37 degrees Celsius). Kcat is 300 min(-1) for dehydrogenase activity with trans-3-hydroxy-2-cyclohexene-1-carboxylate and cis-3- hydroxycyclohexane-1-carboxylic acid as substrates (at pH 7.4 and 37 degrees Celsius). Kcat is 180 min(-1) for dehydrogenase activity with methyl-3-hydroxy-1,4-cyclohexadiene-1-carboxylate as substrate (at pH 7.4 and 37 degrees Celsius). Kcat is 60 min(-1) for dehydrogenase activity with trans-3,5-cyclohexadiene-1,2-diol and 2-cyclohexen-1-ol as substrates (at pH 7.4 and 37 degrees Celsius). Kcat is 44 min(-1) for dehydrogenase activity with trans-3-hydroxycyclohexane-1- carboxylic acid as substrate (at pH 7.4 and 37 degrees Celsius). {ECO:0000269|PubMed:2144454};

Pathway: Siderophore biosynthesis; enterobactin biosynthesis. {ECO:0000305}.

Subunit: Homotetramer; dimer of dimers. EntA and EntE interact together. {ECO:0000269|PubMed:16790929, ECO:0000269|PubMed:21166461, ECO:0000269|PubMed:2521622}.

Induction: Under conditions of iron deficiency and by the fur protein. {ECO:0000269|PubMed:2521621}.

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. {ECO:0000305}.

Sequence caution: Sequence=AAB40796.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};

Annotations taken from UniProtKB at the EBI.


Organism:		Escherichia coli (strain K12).
Taxonomy:		Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia.



Function:		Involved in the biosynthesis of the siderophore enterobactin (enterochelin), which is a macrocyclic trimeric lactone of N-(2,3- dihydroxybenzoyl)-serine. Catalyzes the reversible NAD-dependent oxidation of the C3-hydroxyl group of 2,3-dihydro-2,3-dihydroxybenzoate (2,3-diDHB), producing the transient intermediate 2-hydroxy-3-oxo-4,6- cyclohexadiene-1-carboxylate, which undergoes rapid aromatization to the final product, 2,3-dihydroxybenzoate (2,3-DHB). Only the compounds with a C3-hydroxyl group such as methyl 2,3-dihydro-2,3- dihydroxybenzoate, methyl-3-hydroxy-1,4-cyclohexadiene-1-carboxylate, trans-3-hydroxy-2-cyclohexene-1-carboxylate, cis-3-hydroxy-4- cyclohexene-1-carboxylate, cis-3-hydroxycyclohexane-1-carboxylic acid are oxidized to the corresponding ketone products. The stereospecificity of the C3 allylic alcohol group oxidation is 3R in a 1R,3R dihydro substrate. It can also increase the DHB-AMP ligase activity of EntE by interaction EntE. {ECO:0000269\|PubMed:21166461, ECO:0000269\|PubMed:2144454, ECO:0000269\|PubMed:2521622}.


Catalytic activity:		Reaction=(2S,3S)-2,3-dihydroxy-2,3-dihydrobenzoate + NAD(+) = 2,3- dihydroxybenzoate + H(+) + NADH; Xref=Rhea:RHEA:23824, ChEBI:CHEBI:15378, ChEBI:CHEBI:36654, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:58764; EC=1.3.1.28; Evidence={ECO:0000269\|PubMed:2144454};
Activity regulation:		Inhibited by cis-2-hydroxy-3-cyclohexen-1- carboxylate, cis-2-hydroxycyclohexane-1-carboxylate and trans-2- hydroxycyclohexane-1-carboxylate. {ECO:0000269\|PubMed:2144454}.
Biophysicochemical properties:		Kinetic parameters: KM=0.26 mM for methyl 2,3-dihydro-2,3-dihydroxybenzoate (at pH 7.4 and 37 degrees Celsius) {ECO:0000269\|PubMed:2144454}; KM=0.3 mM for 2,3-dihydro-2,3-dihydroxybenzoate (at pH 7.4 and 37 degrees Celsius) {ECO:0000269\|PubMed:2144454}; KM=1.7 mM for methyl-3-hydroxy-1,4-cyclohexadiene-1-carboxylate (at pH 7.4 and 37 degrees Celsius) {ECO:0000269\|PubMed:2144454}; KM=1.9 mM for trans-3-hydroxy-2-cyclohexene-1-carboxylate (at pH 7.4 and 37 degrees Celsius) {ECO:0000269\|PubMed:2144454}; KM=2.8 mM for cis-3-hydroxy-4-cyclohexene-1-carboxylate (at pH 7.4 and 37 degrees Celsius) {ECO:0000269\|PubMed:2144454}; KM=4.1 mM for cis-3-hydroxycyclohexane-1-carboxylic acid (at pH 7.4 and 37 degrees Celsius) {ECO:0000269\|PubMed:2144454}; KM=16.5 mM for trans-3,5-cyclohexadiene-1,2-diol (at pH 7.4 and 37 degrees Celsius) {ECO:0000269\|PubMed:2144454}; KM=25.2 mM for trans-3-hydroxycyclohexane-1-carboxylic acid (at pH 7.4 and 37 degrees Celsius) {ECO:0000269\|PubMed:2144454}; KM=83.3 mM for 2-cyclohexen-1-ol (at pH 7.4 and 37 degrees Celsius) {ECO:0000269\|PubMed:2144454}; KM=168 mM for cis-3,5-cyclohexadiene-1,2-diol (at pH 7.4 and 37 degrees Celsius) {ECO:0000269\|PubMed:2144454}; Note=Kcat is 5550 min(-1) for dehydrogenase activity with 2,3- dihydro-2,3-dihydroxybenzoate as substrate (at pH 7.4 and 37 degrees Celsius). Kcat is 1380 min(-1) for dehydrogenase activity with cis-3- hydroxy-4-cyclohexene-1-carboxylate as substrate (at pH 7.4 and 37 degrees Celsius). Kcat is 1050 min(-1) for dehydrogenase activity with 2,3-dihydro-2,3-dihydroxybenzoate as substrate (at pH 7.4 and 37 degrees Celsius). Kcat is 1000 min(-1) for dehydrogenase activity with cis-3,5-cyclohexadiene-1,2-diol as substrate (at pH 7.4 and 37 degrees Celsius). Kcat is 300 min(-1) for dehydrogenase activity with trans-3-hydroxy-2-cyclohexene-1-carboxylate and cis-3- hydroxycyclohexane-1-carboxylic acid as substrates (at pH 7.4 and 37 degrees Celsius). Kcat is 180 min(-1) for dehydrogenase activity with methyl-3-hydroxy-1,4-cyclohexadiene-1-carboxylate as substrate (at pH 7.4 and 37 degrees Celsius). Kcat is 60 min(-1) for dehydrogenase activity with trans-3,5-cyclohexadiene-1,2-diol and 2-cyclohexen-1-ol as substrates (at pH 7.4 and 37 degrees Celsius). Kcat is 44 min(-1) for dehydrogenase activity with trans-3-hydroxycyclohexane-1- carboxylic acid as substrate (at pH 7.4 and 37 degrees Celsius). {ECO:0000269\|PubMed:2144454};
Pathway:		Siderophore biosynthesis; enterobactin biosynthesis. {ECO:0000305}.
Subunit:		Homotetramer; dimer of dimers. EntA and EntE interact together. {ECO:0000269\|PubMed:16790929, ECO:0000269\|PubMed:21166461, ECO:0000269\|PubMed:2521622}.
Induction:		Under conditions of iron deficiency and by the fur protein. {ECO:0000269\|PubMed:2521621}.
Similarity:		Belongs to the short-chain dehydrogenases/reductases (SDR) family. {ECO:0000305}.
Sequence caution:		Sequence=AAB40796.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};