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UniProt functional annotation for Q9RC92 | ||
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| UniProt code: Q9RC92. |
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| Organism: | |
Bacillus sp. (strain GL1). |
| Taxonomy: | |
Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus. |
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| Function: | |
Catalyzes the hydrolysis of oligosaccharides with unsaturated glucuronyl residues at the non-reducing terminal, to a sugar or an amino sugar, and an unsaturated D-glucuronic acid (GlcA), which is nonenzymatically converted immediately to alpha-keto acid. {ECO:0000269|PubMed:10441389, ECO:0000269|PubMed:21147778}. |
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| Catalytic activity: | |
Reaction=beta-D-Delta(4)-GlcA-(1->4)-beta-D-Glc-(1->4)-alpha-L-Rha- (1->3)-D-Glc + H2O = 5-dehydro-4-deoxy-D-glucuronate + beta-D-Glc- (1->4)-alpha-L-Rha-(1->3)-D-Glc; Xref=Rhea:RHEA:31635, ChEBI:CHEBI:15377, ChEBI:CHEBI:17117, ChEBI:CHEBI:134389, ChEBI:CHEBI:134390; EC=3.2.1.179; Evidence={ECO:0000269|PubMed:10441389, ECO:0000269|PubMed:21147778}; |
| Activity regulation: | |
Partially inhibited by divalent metal ions such as calcium, copper, iron and mercury. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=0.381 mM for unsaturated chondroitin disaccharidee (delta0S) {ECO:0000269|PubMed:10441389, ECO:0000269|PubMed:21147778}; KM=18.6 mM for unsaturated chondroitin disaccharide sulfated at C-6 position of GalNAc residue (delta6S) {ECO:0000269|PubMed:10441389, ECO:0000269|PubMed:21147778}; KM=90 uM for gellan lyase product (at 30 degrees Celsius and pH 6.5) {ECO:0000269|PubMed:10441389, ECO:0000269|PubMed:21147778}; KM=200 uM for chondroitin lyase product (at 30 degrees Celsius and pH 6.5) {ECO:0000269|PubMed:10441389, ECO:0000269|PubMed:21147778}; KM=226 uM for hyaluronate lyase product (at 30 degrees Celsius and pH 6.5) {ECO:0000269|PubMed:10441389, ECO:0000269|PubMed:21147778}; Note=kcat is 14.1 sec(-1) with unsaturated chondroitin (delta0S). kcat is 54.9 sec(-1) with unsaturated chondroitin disaccharide sulfated at C-6 position of GalNAc residue (delta6S). {ECO:0000269|PubMed:21147778}; pH dependence: Optimum pH is 6.0-6.5. {ECO:0000269|PubMed:10441389, ECO:0000269|PubMed:21147778}; Temperature dependence: Optimum temperature is 45 degrees Celsius. {ECO:0000269|PubMed:10441389, ECO:0000269|PubMed:21147778}; |
| Subunit: | |
Monomer. {ECO:0000269|PubMed:10441389, ECO:0000269|PubMed:16893885}. |
| Subcellular location: | |
Cytoplasm. |
| Similarity: | |
Belongs to the glycosyl hydrolase 88 family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.