PDBsum entry 2ftk

Transferase

PDB id: 2ftk

Contents

Protein chains

181 a.a.

119 a.a.

Metals

_MG ×4

References listed in PDB file

Key reference

• Title: The crystal structure of beryllofluoride spo0f in complex with the phosphotransferase spo0b represents a phosphotransfer pretransition state.
• Authors: K.I.Varughese, I.Tsigelny, H.Zhao.
• Ref.: J Bacteriol, 2006, 188, 4970-4977.
• PubMed id: 16788205

Abstract

A number of regulatory circuits in biological systems function through the exchange of phosphoryl groups from one protein to another. Spo0F and Spo0B are components of a phosphorelay that control sporulation in the bacterium Bacillus subtilis through the exchange of a phosphoryl group. Using beryllofluoride as a mimic for phosphorylation, we trapped the interaction of the phosphorylated Spo0F with Spo0B in the crystal lattice. The transition state of phosphoryl transfer continues to be a highly debated issue, as to whether it is associative or dissociative in nature. The geometry of Spo0F binding to Spo0B favors an associative mechanism for phosphoryl transfer. In order to visualize the autophosphorylation of the histidine kinase, KinA, and the subsequent phosphoryl transfer to Spo0F, we generated in silico models representing these reaction steps.