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PDBsum entry 2fqe
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Oxidoreductase
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PDB id
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2fqe
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References listed in PDB file
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Key reference
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Title
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Crystal structures of e. Coli laccase cueo at different copper concentrations.
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Authors
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X.Li,
Z.Wei,
M.Zhang,
X.Peng,
G.Yu,
M.Teng,
W.Gong.
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Ref.
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Biochem Biophys Res Commun, 2007,
354,
21-26.
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PubMed id
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Abstract
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CueO protein is a hypothetical bacterial laccase and a good laccase candidate
for large scale industrial application. Four CueO crystal structures were
determined at different copper concentrations. Low copper occupancy in apo-CueO
and slow copper reconstitution process in CueO with exogenous copper were
demonstrated. These observations well explain the copper dependence of CueO
oxidase activity. Structural comparison between CueO and other three fungal
laccase proteins indicates that Glu106 in CueO constitutes the primary
counter-work for reconstitution of the trinuclear copper site. Mutation of
Glu106 to a Phe enhanced CueO oxidation activity and supported this hypothesis.
In addition, an extra alpha-helix from Leu351 to Gly378 covers substrate biding
pocket of CueO and might compromises the electron transfer from substrate to
type I copper.
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