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UniProt functional annotation for Q9S5G5 | ||
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| UniProt code: Q9S5G5. |
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| Organism: | |
Escherichia coli O157:H7. |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
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| Catalytic activity: | |
Reaction=D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol- 4-yl)-2-oxopropyl phosphate + H2O; Xref=Rhea:RHEA:11040, ChEBI:CHEBI:15377, ChEBI:CHEBI:57766, ChEBI:CHEBI:58278; EC=4.2.1.19; Evidence={ECO:0000255|HAMAP-Rule:MF_01022}; |
| Catalytic activity: | |
Reaction=H2O + L-histidinol phosphate = L-histidinol + phosphate; Xref=Rhea:RHEA:14465, ChEBI:CHEBI:15377, ChEBI:CHEBI:43474, ChEBI:CHEBI:57699, ChEBI:CHEBI:57980; EC=3.1.3.15; Evidence={ECO:0000255|HAMAP-Rule:MF_01022, ECO:0000269|PubMed:16966333}; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000255|HAMAP-Rule:MF_01022, ECO:0000269|PubMed:16966333}; Note=Binds 2 Mg(2+) ions. Also can use Co(2+) and Mn(2+)ions. {ECO:0000269|PubMed:16966333}; |
| Cofactor: | |
Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000255|HAMAP-Rule:MF_01022, ECO:0000269|PubMed:16966333}; |
| Activity regulation: | |
Inhibited by Ca(2+). {ECO:0000269|PubMed:16966333}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=54 uM for histidinol phosphate (in the presence of Mg(2+)) {ECO:0000269|PubMed:16966333}; KM=54 uM for histidinol phosphate (in the presence of Co(2+)) {ECO:0000269|PubMed:16966333}; KM=41 uM for histidinol phosphate (in the presence of Zn(2+)) {ECO:0000269|PubMed:16966333}; KM=52 uM for histidinol phosphate (in the presence of Mn(2+)) {ECO:0000269|PubMed:16966333}; |
| Pathway: | |
Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 6/9. {ECO:0000255|HAMAP-Rule:MF_01022}. |
| Pathway: | |
Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 8/9. {ECO:0000255|HAMAP-Rule:MF_01022}. |
| Subcellular location: | |
Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01022}. |
| Similarity: | |
In the N-terminal section; belongs to the histidinol- phosphatase family. {ECO:0000255|HAMAP-Rule:MF_01022}. |
| Similarity: | |
In the C-terminal section; belongs to the imidazoleglycerol-phosphate dehydratase family. {ECO:0000255|HAMAP- Rule:MF_01022}. |
| Sequence caution: | |
Sequence=AAG57081.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=BAB36246.1; Type=Erroneous initiation; Evidence={ECO:0000305}; |
Annotations taken from UniProtKB at the EBI.