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UniProt functional annotation for P53590 | ||
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| UniProt code: P53590. |
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| Organism: | |
Sus scrofa (Pig). |
| Taxonomy: | |
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus. |
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| Function: | |
GTP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit. {ECO:0000255|HAMAP-Rule:MF_03221, ECO:0000269|PubMed:27487822, ECO:0000269|PubMed:8401211}. |
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| Catalytic activity: | |
Reaction=CoA + GTP + succinate = GDP + phosphate + succinyl-CoA; Xref=Rhea:RHEA:22120, ChEBI:CHEBI:30031, ChEBI:CHEBI:37565, ChEBI:CHEBI:43474, ChEBI:CHEBI:57287, ChEBI:CHEBI:57292, ChEBI:CHEBI:58189; EC=6.2.1.4; Evidence={ECO:0000255|HAMAP- Rule:MF_03221, ECO:0000269|PubMed:27487822, ECO:0000269|PubMed:8401211}; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000255|HAMAP-Rule:MF_03221}; Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_03221}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=0.76 mM for succinate {ECO:0000269|PubMed:27487822}; Vmax=5.99 umol/min/mg enzyme {ECO:0000269|PubMed:27487822}; |
| Pathway: | |
Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1. {ECO:0000255|HAMAP- Rule:MF_03221, ECO:0000305|PubMed:8401211}. |
| Subunit: | |
Heterodimer of an alpha and a beta subunit. The beta subunit determines specificity for GTP. {ECO:0000255|HAMAP-Rule:MF_03221, ECO:0000269|PubMed:10873456, ECO:0000269|PubMed:16481318, ECO:0000269|PubMed:26249701, ECO:0000269|PubMed:27487822}. |
| Subcellular location: | |
Mitochondrion {ECO:0000255|HAMAP-Rule:MF_03221}. |
| Similarity: | |
Belongs to the succinate/malate CoA ligase beta subunit family. GTP-specific subunit beta subfamily. {ECO:0000255|HAMAP- Rule:MF_03221}. |
| Sequence caution: | |
Sequence=AAA31120.1; Type=Erroneous initiation; Evidence={ECO:0000305}; |
Annotations taken from UniProtKB at the EBI.