UniProt functional annotation for P9WIB9



	UniProt functional annotation for P9WIB9

UniProt code: P9WIB9.

Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).

Taxonomy: Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex.

Function: Catalyzes the Claisen rearrangement of chorismate to prephenate. May play some role in the pathogenicity. {ECO:0000269|PubMed:15654876, ECO:0000269|PubMed:15737998}.

Catalytic activity: Reaction=chorismate = prephenate; Xref=Rhea:RHEA:13897, ChEBI:CHEBI:29748, ChEBI:CHEBI:29934; EC=5.4.99.5;

Activity regulation: Tyrosine, phenylalanine, and tryptophan moderately enhance chorismate mutase activity at low concentrations, but allosterically inhibit the enzyme at higher concentrations. {ECO:0000269|PubMed:15737998}.

Biophysicochemical properties: Kinetic parameters: KM=120 uM for chorismate (at pH 7.5 and 37 degrees Celsius) {ECO:0000269|PubMed:15737998}; KM=180 uM for chorismate (at pH 7.5 and 30 degrees Celsius) {ECO:0000269|PubMed:15654876}; KM=500 uM for chorismate (with 27.5 nM of protein at pH 7.5 and 37 degrees Celsius) {ECO:0000269|PubMed:17146044}; KM=670 uM for chorismate (with 8 nM of protein at pH 7.5 and 37 degrees Celsius) {ECO:0000269|PubMed:17146044}; Vmax=74 umol/min/mg enzyme (at pH 7.5 and 37 degrees Celsius) {ECO:0000269|PubMed:15737998}; pH dependence: Optimum pH is 7.5. The activity is dramatically affected under alkaline conditions (pH 9.5). {ECO:0000269|PubMed:15654876, ECO:0000269|PubMed:15737998}; Temperature dependence: Optimum temperature is between 37 to 50 degrees Celsius. An increase in temperature does increase enzyme activity, and complete reversible denaturation of the enzyme occurs at a temperature close to 60 degrees Celsius. {ECO:0000269|PubMed:15654876, ECO:0000269|PubMed:15737998};

Pathway: Metabolic intermediate biosynthesis; prephenate biosynthesis; prephenate from chorismate: step 1/1.

Subunit: Homodimer. {ECO:0000269|PubMed:15654876, ECO:0000269|PubMed:15737998, ECO:0000269|PubMed:16499927, ECO:0000269|PubMed:16752890, ECO:0000269|PubMed:17146044}.

Subcellular location: Secreted {ECO:0000269|PubMed:15654876, ECO:0000269|PubMed:15737998, ECO:0000269|PubMed:17146044}.

Miscellaneous: Was identified as a high-confidence drug target. {ECO:0000305|PubMed:19099550}.

Miscellaneous: In the presence of high concentrations of tyrosine, phenylalanine, and tryptophan, the enzyme is completely protected from proteolytic degradation.

Annotations taken from UniProtKB at the EBI.


Organism:		Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
Taxonomy:		Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex.



Function:		Catalyzes the Claisen rearrangement of chorismate to prephenate. May play some role in the pathogenicity. {ECO:0000269\|PubMed:15654876, ECO:0000269\|PubMed:15737998}.


Catalytic activity:		Reaction=chorismate = prephenate; Xref=Rhea:RHEA:13897, ChEBI:CHEBI:29748, ChEBI:CHEBI:29934; EC=5.4.99.5;
Activity regulation:		Tyrosine, phenylalanine, and tryptophan moderately enhance chorismate mutase activity at low concentrations, but allosterically inhibit the enzyme at higher concentrations. {ECO:0000269\|PubMed:15737998}.
Biophysicochemical properties:		Kinetic parameters: KM=120 uM for chorismate (at pH 7.5 and 37 degrees Celsius) {ECO:0000269\|PubMed:15737998}; KM=180 uM for chorismate (at pH 7.5 and 30 degrees Celsius) {ECO:0000269\|PubMed:15654876}; KM=500 uM for chorismate (with 27.5 nM of protein at pH 7.5 and 37 degrees Celsius) {ECO:0000269\|PubMed:17146044}; KM=670 uM for chorismate (with 8 nM of protein at pH 7.5 and 37 degrees Celsius) {ECO:0000269\|PubMed:17146044}; Vmax=74 umol/min/mg enzyme (at pH 7.5 and 37 degrees Celsius) {ECO:0000269\|PubMed:15737998}; pH dependence: Optimum pH is 7.5. The activity is dramatically affected under alkaline conditions (pH 9.5). {ECO:0000269\|PubMed:15654876, ECO:0000269\|PubMed:15737998}; Temperature dependence: Optimum temperature is between 37 to 50 degrees Celsius. An increase in temperature does increase enzyme activity, and complete reversible denaturation of the enzyme occurs at a temperature close to 60 degrees Celsius. {ECO:0000269\|PubMed:15654876, ECO:0000269\|PubMed:15737998};
Pathway:		Metabolic intermediate biosynthesis; prephenate biosynthesis; prephenate from chorismate: step 1/1.
Subunit:		Homodimer. {ECO:0000269\|PubMed:15654876, ECO:0000269\|PubMed:15737998, ECO:0000269\|PubMed:16499927, ECO:0000269\|PubMed:16752890, ECO:0000269\|PubMed:17146044}.
Subcellular location:		Secreted {ECO:0000269\|PubMed:15654876, ECO:0000269\|PubMed:15737998, ECO:0000269\|PubMed:17146044}.
Miscellaneous:		Was identified as a high-confidence drug target. {ECO:0000305\|PubMed:19099550}.
Miscellaneous:		In the presence of high concentrations of tyrosine, phenylalanine, and tryptophan, the enzyme is completely protected from proteolytic degradation.