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PDBsum entry 2fe3
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DNA binding protein
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PDB id
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2fe3
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References listed in PDB file
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Key reference
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Title
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Crystal structure of the apo-Perr-Zn protein from bacillus subtilis.
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Authors
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D.A.Traoré,
A.El ghazouani,
S.Ilango,
J.Dupuy,
L.Jacquamet,
J.L.Ferrer,
C.Caux-Thang,
V.Duarte,
J.M.Latour.
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Ref.
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Mol Microbiol, 2006,
61,
1211-1219.
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PubMed id
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Abstract
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Bacteria adapt to elevated levels of Reactive Oxygen Species (ROS) by increasing
the expression of defence and repair proteins, which is regulated by ROS
responsive transcription factors. In Bacillus subtilis the zinc protein PerR, a
peroxide sensor that binds DNA in the presence of a regulatory metal Mn2+ or
Fe2+, mediates the adaptive response to H2O2. This study presents the first
crystal structure of apo-PerR-Zn which shows that all four cysteine residues of
the protein are involved in zinc co-ordination. The Zn(Cys)4 site locks the
dimerization domain and stabilizes the dimer. Sequence alignment of PerR-like
proteins supports that this structural site may constitute a distinctive feature
of this class of peroxide stress regulators.
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