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PDBsum entry 2fdn
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Electron transport
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PDB id
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2fdn
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References listed in PDB file
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Key reference
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Title
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Atomic resolution (0.94 a) structure of clostridium acidurici ferredoxin. Detailed geometry of [4fe-4s] clusters in a protein.
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Authors
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Z.Dauter,
K.S.Wilson,
L.C.Sieker,
J.Meyer,
J.M.Moulis.
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Ref.
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Biochemistry, 1997,
36,
16065-16073.
[DOI no: ]
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PubMed id
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Abstract
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The crystal structure of the 2[4Fe-4S] ferredoxin from Clostridium acidurici has
been solved using X-ray diffraction data extending to atomic resolution, 0.94 A,
recorded at 100 K. The model was refined with anisotropic representation of
atomic displacement parameters for all non-hydrogen atoms and with hydrogens
riding on their parent atoms. Stereochemical restraints were applied to the
protein chain but not to the iron-sulfur clusters. The final R factor is 10.03 %
for all data. Inversion of the final least-squares matrix allowed direct
estimation of the errors of individual parameters. The estimated errors in
positions for protein main chain atoms are below 0.02 A and about 0.003 A for
the heavier [4Fe-4S] cluster atoms. Significant differences between the
stereochemistry of the two clusters and distortion of both of them from ideal Td
tetrahedral symmetry can be defined in detail at this level of accuracy. Regions
of alternative conformations include not only protein side chains but also two
regions of the main chain. One such region is the loop of residues 25-29, which
was highly disordered in the room temperature structure.
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Secondary reference #1
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Title
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Refined crystal structure of the 2[4fe-4s] ferredoxin from clostridium acidurici at 1.84 a resolution.
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Authors
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E.D.Duée,
E.Fanchon,
J.Vicat,
L.C.Sieker,
J.Meyer,
J.M.Moulis.
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Ref.
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J Mol Biol, 1994,
243,
683-695.
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PubMed id
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