PDBsum entry 2fd2

Electron transfer(iron-sulfur protein)

PDB id: 2fd2

Contents

Protein chain

106 a.a.

Ligands

SF4

F3S

Waters ×30

References listed in PDB file

Key reference

Title

Crystallographic analysis of two site-Directed mutants of azotobacter vinelandii ferredoxin.

Authors

J.Soman, S.Iismaa, C.D.Stout.

Ref.

J Biol Chem, 1991, 266, 21558-21562.

PubMed id

1939185

Note In the PDB file this reference is annotated as "TO BE PUBLISHED". The citation details given above were identified by an automated search of PubMed on title and author names, giving a perfect match.

Abstract

The crystal structure of the C24A mutant of Azotobacter vinelandii 7Fe ferredoxin (FdI) has been solved and refined at 2.0-A resolution. The structure is isomorphous to native FdI except at the site of mutation where A24 moves toward the [4Fe-4S] cluster. In spite of this inefficient packing results: three of five van der Waals contacts from the S gamma of C24 in native FdI are lost and the remaining two become longer. Consequently, the [4Fe-4S] cluster is either disordered or has a higher temperature factor (B factor) compared to the rest of the C24A FdI molecule. In addition, the entire C24A FdI structure has a higher overall B factor than native FdI. Therefore, in comparison to native FdI, the C24A mutant is isomorphous but exhibits large differences in B factor, especially at the [4Fe-4S] cluster. In contrast, the C20A FdI structure (Martin, A. G., Burgess, B. K., Stout, C. D., Cash, V. L., Dean, D. R., Jensen, G. M., and Stephens, P. J. (1990) Proc. Natl. Acad. Sci. U. S. A. 87, 598-602), which contains large structural rearrangements in the vicinity of the [4Fe-4S] cluster, exhibits essentially no change in B factor. The conformational change observed at residue 24 is similar in both C24A and C20A FdI structures. The solvent accessibility of the Fe atoms in the [3Fe-4S] and [4Fe-4S] clusters is similar in C24A, C20A, and native FdI.

Secondary reference #1

Title

Site-Directed mutagenesis of azotobacter vinelandii ferredoxin i: [fe-S] cluster-Driven protein rearrangement.

Authors

A.E.Martín, B.K.Burgess, C.D.Stout, V.L.Cash, D.R.Dean, G.M.Jensen, P.J.Stephens.

Ref.

Proc Natl Acad Sci U S A, 1990, 87, 598-602. [DOI no: 10.1073/pnas.87.2.598]

PubMed id

2153958

Secondary reference #2

Title

Refinement of the 7 fe ferredoxin from azotobacter vinelandii at 1.9 a resolution.

Author

C.D.Stout.

Ref.

J Mol Biol, 1989, 205, 545-555.

PubMed id

2926817

Secondary reference #3

Title

7-Iron ferredoxin revisited.

Author

C.D.Stout.

Ref.

J Biol Chem, 1988, 263, 9256-9260.

PubMed id

3379067

Secondary reference #4

Title

[4fe-4s]-Cluster-Depleted azotobacter vinelandii ferredoxin i: a new 3fe iron-Sulfur protein.

Authors

P.J.Stephens, T.V.Morgan, F.Devlin, J.E.Penner-Hahn, K.O.Hodgson, R.A.Scott, C.D.Stout, B.K.Burgess.

Ref.

Proc Natl Acad Sci U S A, 1985, 82, 5661-5665. [DOI no: 10.1073/pnas.82.17.5661]

PubMed id

2994040

Secondary reference #5

Title

Structure of azotobacter vinelandii 7fe ferredoxin. Amino acid sequence and electron density maps of residues.

Authors

J.B.Howard, T.W.Lorsbach, D.Ghosh, K.Melis, C.D.Stout.

Ref.

J Biol Chem, 1983, 258, 508-522.

PubMed id

6848518

Secondary reference #6

Title

Iron-Sulfur clusters and protein structure of azotobacter ferredoxin at 2.0 a resolution.

Authors

D.Ghosh, S.O'Donnell, W.Furey, A.H.Robbins, C.D.Stout.

Ref.

J Mol Biol, 1982, 158, 73. [DOI no: 10.1016/0022-2836(82)90451-X]

PubMed id

7120409

Figure 1.
FIG:. 1.5. Opposite view from Fig. 14 showing glutamate 18 and 0x0 in relation to Fr(2) of the [3Fe-3SI luster and adjacent water molecules within 45 A of 0x0. 0, 37 and 044,, are within 35 A of 0x0. Watrr oxygens in adition to 0x0 arr numbered from 109 to 451.

Figure 9.
FIG:. 9. (`o-ordintes of` [4Fr-4SJ(S,), cluster in t,hth tinal model

The above figures are reproduced from the cited reference with permission from Elsevier

Secondary reference #7

Title

Structure of a 7fe ferredoxin from azotobacter vinelandii.

Authors

D.Ghosh, W.Furey, S.O'Donnell, C.D.Stout.

Ref.

J Biol Chem, 1981, 256, 4185-4192.

PubMed id

7217079

Secondary reference #8

Title

Iron-Sulfur clusters in azotobacter ferredoxin at 2.5 a resolution.

Authors

C.D.Stout, D.Ghosh, V.Pattabhi, A.H.Robbins.

Ref.

J Biol Chem, 1980, 255, 1797-1800.

PubMed id

7354058

Secondary reference #9

Title

Structure of the iron-Sulfur clusters in azotobacter ferredoxin at 4.0 angstroms resolution

Author

C.D.Stout.

Ref.

am cryst assoc ,abstr papers, 1979, 6, 97.

Secondary reference #10

Title

Two crystal forms of azotobacter ferredoxin.

Author

C.D.Stout.

Ref.

J Biol Chem, 1979, 254, 3598-3599.

PubMed id

429371

Secondary reference #11

Title

Structure of the iron-Sulphur clusters in azotobacter ferredoxin at 4.0 a resolution.

Author

C.D.Stout.

Ref.

Nature, 1979, 279, 83-84.

PubMed id

450082