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UniProt functional annotation for Q8I914 | ||
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| UniProt code: Q8I914. |
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| Organism: | |
Loxosceles laeta (South American recluse spider) (Scytodes laeta). |
| Taxonomy: | |
Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae; Araneomorphae; Haplogynae; Scytodoidea; Sicariidae; Loxosceles. |
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| Function: | |
Dermonecrotic toxins cleave the phosphodiester linkage between the phosphate and headgroup of certain phospholipids (sphingolipid and lysolipid substrates), forming an alcohol (often choline) and a cyclic phosphate (By similarity). This toxin acts on sphingomyelin (SM) with high activity (PubMed:12419302, PubMed:14732720). It also act on acyl- and alkyl- lysophosphatidylcholine (LPC), but not on sphingosylphosphorylcholine (SPC) and phosphatidylcholine (PC) (PubMed:14732720). It may also act on ceramide phosphoethanolamine (CPE), and lysophosphatidylethanolamine (LPE), but not on lysophosphatidylserine (LPS), and lysophosphatidylglycerol (LPG) (By similarity). It acts by transphosphatidylation, releasing exclusively cyclic phosphate products as second products (By similarity). Induces complement-dependent hemolysis and dermonecrosis (PubMed:12419302). Also induces increased vascular permeability, edema, inflammatory response, and platelet aggregation (By similarity). {ECO:0000250|UniProtKB:A0A0D4WTV1, ECO:0000250|UniProtKB:P0CE80, ECO:0000269|PubMed:12419302, ECO:0000269|PubMed:14732720}. |
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| Catalytic activity: | |
Reaction=an N-(acyl)-sphingosylphosphocholine = an N-(acyl)-sphingosyl- 1,3-cyclic phosphate + choline; Xref=Rhea:RHEA:60652, ChEBI:CHEBI:15354, ChEBI:CHEBI:64583, ChEBI:CHEBI:143892; Evidence={ECO:0000305|PubMed:12419302, ECO:0000305|PubMed:14732720}; |
| Catalytic activity: | |
Reaction=an N-(acyl)-sphingosylphosphoethanolamine = an N-(acyl)- sphingosyl-1,3-cyclic phosphate + ethanolamine; Xref=Rhea:RHEA:60648, ChEBI:CHEBI:57603, ChEBI:CHEBI:143891, ChEBI:CHEBI:143892; Evidence={ECO:0000250|UniProtKB:A0A0D4WTV1}; |
| Catalytic activity: | |
Reaction=a 1-acyl-sn-glycero-3-phosphocholine = a 1-acyl-sn-glycero- 2,3-cyclic phosphate + choline; Xref=Rhea:RHEA:60700, ChEBI:CHEBI:15354, ChEBI:CHEBI:58168, ChEBI:CHEBI:143947; Evidence={ECO:0000305|PubMed:14732720}; |
| Catalytic activity: | |
Reaction=a 1-acyl-sn-glycero-3-phosphoethanolamine = a 1-acyl-sn- glycero-2,3-cyclic phosphate + ethanolamine; Xref=Rhea:RHEA:60704, ChEBI:CHEBI:57603, ChEBI:CHEBI:64381, ChEBI:CHEBI:143947; Evidence={ECO:0000250|UniProtKB:A0A0D4WTV1}; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:14732720, ECO:0000269|PubMed:15654080, ECO:0000269|PubMed:16480957, ECO:0000312|PDB:1XX1, ECO:0000312|PDB:2F9R}; Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000269|PubMed:14732720, ECO:0000269|PubMed:15654080, ECO:0000269|PubMed:16480957, ECO:0000312|PDB:1XX1, ECO:0000312|PDB:2F9R}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=44.4 uM for 1-oleoyl-LPC {ECO:0000269|PubMed:14732720}; Vmax=212 nmol/min/mg enzyme toward 1-oleoyl-LPC {ECO:0000269|PubMed:14732720}; |
| Subcellular location: | |
Secreted {ECO:0000305|PubMed:12419302}. |
| Tissue specificity: | |
Expressed by the venom gland. {ECO:0000305|PubMed:12419302}. |
| Similarity: | |
Belongs to the arthropod phospholipase D family. Class I subfamily. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.