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PDBsum entry 2f77
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Viral protein
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PDB id
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2f77
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References listed in PDB file
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Key reference
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Title
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D-Retrovirus morphogenetic switch driven by the targeting signal accessibility to tctex-1 of dynein.
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Authors
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J.Vlach,
J.Lipov,
M.Rumlová,
V.Veverka,
J.Lang,
P.Srb,
Z.Knejzlík,
I.Pichová,
E.Hunter,
R.Hrabal,
T.Ruml.
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Ref.
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Proc Natl Acad Sci U S A, 2008,
105,
10565-10570.
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PubMed id
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Abstract
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Despite extensive data demonstrating that immature retroviral particle assembly
can take place either at the plasma membrane or at a distinct location within
the cytoplasm, targeting of viral precursor proteins to either assembly site
still remains poorly understood. Biochemical data presented here suggest that
Tctex-1, a light chain of the molecular motor dynein, is involved in the
intracellular targeting of Mason-Pfizer monkey virus (M-PMV) polyproteins to the
cytoplasmic assembly site. Comparison of the three-dimensional structures of
M-PMV wild-type matrix protein (wt MA) with a single amino acid mutant (R55F),
which redirects assembly from a cytoplasmic site to the plasma membrane,
revealed different mutual orientations of their C- and N-terminal domains. This
conformational change buries a putative intracellular targeting motif located
between both domains in the hydrophobic pocket of the MA molecule, thereby
preventing the interaction with cellular transport mechanisms.
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