PDBsum entry 2f2h

Hydrolase

PDB id: 2f2h

Contents

Protein chains

(+ 0 more) 773 a.a.

Ligands

SO4 ×9

XTG ×7

GOL ×14

MPO ×5

Waters ×2003

References listed in PDB file

Key reference

• Title: Expanding the thioglycoligase strategy to the synthesis of alpha-Linked thioglycosides allows structural investigation of the parent enzyme/substrate complex.
• Authors: Y.W.Kim, A.L.Lovering, H.Chen, T.Kantner, L.P.Mcintosh, N.C.Strynadka, S.G.Withers.
• Ref.: J Am Chem Soc, 2006, 128, 2202-2203. [DOI no: 10.1021/ja057904a]
• PubMed id: 16478160

Abstract

For the first time, the thioglycoligase strategy has been successfully applied to alpha-glycosidases. The alpha-thioglycoligases derived from the family 31 glycosidases, alpha-xylosidase from E. coli (YicI) and alpha-glucosidase from Sulfolobus solfataricus, catalyze thioglycoligase reactions using alpha-glycosyl fluorides and deoxythioglycosides as donors and acceptors, respectively, in yields up to 86%. In addition, we describe the Michaelis complex of YicI using one of the thioglycosides as a nonhydrolyzable substrate analogue and discuss the structural insights this yields into the specificity and mechanism of family 31 alpha-glycosidases and the molecular basis of an associated genetic disease.