|
|
||
|
|
|
|
|
UniProt functional annotation for Q9NPJ3 | ||
|
|
|
|
|
|
||
| UniProt code: Q9NPJ3. |
|
||
| Organism: | |
Homo sapiens (Human). |
| Taxonomy: | |
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. |
|
||
|
||
| Function: | |
Catalyzes the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH (PubMed:16934754, PubMed:19170545). Has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates (By similarity) (PubMed:16934754, PubMed:19170545). Can also hydrolyze 3- hydroxyphenylacetyl-CoA and 3,4-dihydroxyphenylacetyl-CoA (in vitro) (By similarity) (PubMed:16934754, PubMed:19170545). May play a role in controlling adaptive thermogenesis (By similarity). {ECO:0000250|UniProtKB:Q9CQR4, ECO:0000269|PubMed:16934754, ECO:0000269|PubMed:19170545}. |
|
||
| Catalytic activity: | |
Reaction=a fatty acyl-CoA + H2O = a fatty acid + CoA + H(+); Xref=Rhea:RHEA:16781, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:28868, ChEBI:CHEBI:57287, ChEBI:CHEBI:77636; Evidence={ECO:0000269|PubMed:19170545}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16782; Evidence={ECO:0000303|PubMed:19170545}; |
| Catalytic activity: | |
Reaction=decanoyl-CoA + H2O = CoA + decanoate + H(+); Xref=Rhea:RHEA:40059, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:27689, ChEBI:CHEBI:57287, ChEBI:CHEBI:61430; Evidence={ECO:0000269|PubMed:19170545}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40060; Evidence={ECO:0000303|PubMed:19170545}; |
| Catalytic activity: | |
Reaction=H2O + octanoyl-CoA = CoA + H(+) + octanoate; Xref=Rhea:RHEA:30143, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:25646, ChEBI:CHEBI:57287, ChEBI:CHEBI:57386; Evidence={ECO:0000269|PubMed:19170545}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:30144; Evidence={ECO:0000303|PubMed:19170545}; |
| Catalytic activity: | |
Reaction=butanoyl-CoA + H2O = butanoate + CoA + H(+); Xref=Rhea:RHEA:40111, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17968, ChEBI:CHEBI:57287, ChEBI:CHEBI:57371; Evidence={ECO:0000269|PubMed:19170545}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40112; Evidence={ECO:0000303|PubMed:19170545}; |
| Catalytic activity: | |
Reaction=H2O + hexanoyl-CoA = CoA + H(+) + hexanoate; Xref=Rhea:RHEA:40115, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17120, ChEBI:CHEBI:57287, ChEBI:CHEBI:62620; Evidence={ECO:0000269|PubMed:19170545}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40116; Evidence={ECO:0000303|PubMed:19170545}; |
| Catalytic activity: | |
Reaction=H2O + tetradecanoyl-CoA = CoA + H(+) + tetradecanoate; Xref=Rhea:RHEA:40119, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30807, ChEBI:CHEBI:57287, ChEBI:CHEBI:57385; Evidence={ECO:0000269|PubMed:19170545}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40120; Evidence={ECO:0000303|PubMed:19170545}; |
| Catalytic activity: | |
Reaction=H2O + hexadecanoyl-CoA = CoA + H(+) + hexadecanoate; Xref=Rhea:RHEA:16645, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:57379; EC=3.1.2.2; Evidence={ECO:0000269|PubMed:19170545}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16646; Evidence={ECO:0000303|PubMed:19170545}; |
| Catalytic activity: | |
Reaction=dodecanoyl-CoA + H2O = CoA + dodecanoate + H(+); Xref=Rhea:RHEA:30135, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:18262, ChEBI:CHEBI:57287, ChEBI:CHEBI:57375; Evidence={ECO:0000269|PubMed:19170545}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:30136; Evidence={ECO:0000303|PubMed:19170545}; |
| Catalytic activity: | |
Reaction=(9Z)-octadecenoyl-CoA + H2O = (9Z)-octadecenoate + CoA + H(+); Xref=Rhea:RHEA:40139, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387; Evidence={ECO:0000269|PubMed:19170545}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40140; Evidence={ECO:0000303|PubMed:19170545}; |
| Catalytic activity: | |
Reaction=(5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + H2O = (5Z,8Z,11Z,14Z)- eicosatetraenoate + CoA + H(+); Xref=Rhea:RHEA:40151, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:32395, ChEBI:CHEBI:57287, ChEBI:CHEBI:57368; Evidence={ECO:0000269|PubMed:19170545}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40152; Evidence={ECO:0000303|PubMed:19170545}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=4.9 uM for n-decanoyl-CoA {ECO:0000269|PubMed:19170545}; KM=26 uM for n-octanoyl-CoA {ECO:0000269|PubMed:19170545}; KM=70 uM for n-hexanoyl-CoA {ECO:0000269|PubMed:19170545}; KM=600 uM for n-butyryl-CoA {ECO:0000269|PubMed:19170545}; KM=220 uM for 2-butenoyl-CoA {ECO:0000269|PubMed:19170545}; KM=120 uM for beta-methylcrotonyl-CoA {ECO:0000269|PubMed:19170545}; KM=180 uM for crotonyl-CoA {ECO:0000269|PubMed:19170545}; KM=250 uM for tiglyl-CoA {ECO:0000269|PubMed:19170545}; KM=190 uM for n-propionyl-CoA {ECO:0000269|PubMed:19170545}; KM=290 uM for acetyl-CoA {ECO:0000269|PubMed:19170545}; KM=9.9 uM for lauroyl-CoA/dodecanoyl-CoA {ECO:0000269|PubMed:19170545}; KM=5 uM for myristoyl-CoA/tetradecanoyl-CoA (at 25 degrees Celsius) {ECO:0000269|PubMed:19170545}; KM=9 uM for myristoyl-CoA/tetradecanoyl-CoA (at 37 degrees Celsius) {ECO:0000269|PubMed:19170545}; KM=16 uM for n-palmitoyl-CoA {ECO:0000269|PubMed:19170545}; KM=9 uM for palmitoleoyl-CoA {ECO:0000269|PubMed:19170545}; KM=20 uM for stearoyl-CoA {ECO:0000269|PubMed:19170545}; KM=9 uM for oleoyl-CoA {ECO:0000269|PubMed:19170545}; KM=310 uM for linoleoyl-CoA {ECO:0000269|PubMed:19170545}; KM=20 uM for arachidonoyl-CoA/(5Z,8Z,11Z,14Z)-eicosatetraenoate {ECO:0000269|PubMed:19170545}; KM=41 uM for 3-hydroxyphenylacetyl-CoA {ECO:0000269|PubMed:19170545}; KM=10 uM for 3,4-dihydroxyphenylacetyl-CoA {ECO:0000269|PubMed:19170545}; KM=25 uM for 3,5-dihydroxyphenylacetyl-CoA {ECO:0000269|PubMed:19170545}; KM=49 uM for 4-hydroxyphenylacetyl-CoA {ECO:0000269|PubMed:19170545}; KM=240 uM for phenylacetyl-CoA {ECO:0000269|PubMed:19170545}; KM=32 uM for 3-hydroxybenzoyl-CoA {ECO:0000269|PubMed:19170545}; KM=13 uM for 4-chlorobenzoyl-CoA {ECO:0000269|PubMed:19170545}; KM=110 uM for beta-hydroxybutyryl-CoA {ECO:0000269|PubMed:19170545}; KM=670 uM for glutaryl-CoA {ECO:0000269|PubMed:19170545}; KM=2900 uM for 3-hydroxy-3-methylglutaryl-CoA {ECO:0000269|PubMed:19170545}; KM=280 uM for malonyl-CoA {ECO:0000269|PubMed:19170545}; KM=210 uM for methylmalonyl-CoA {ECO:0000269|PubMed:19170545}; Note=kcat is 0.016 s(-1) for n-decanoyl-CoA hydrolase activity (PubMed:19170545). kcat is 0.0047 s(-1) for n-octanoyl-CoA hydrolase activity (PubMed:19170545). kcat is 0.022 s(-1) for n-hexanoyl-CoA hydrolase activity (PubMed:19170545). kcat is 0.047 s(-1) for n- butyryl-CoA hydrolase activity (PubMed:19170545). kcat is 0.014 s(-1) for 2-butenoyl-CoA hydrolase activity (PubMed:19170545). kcat is 0.023 s(-1) for beta-methylcrotonyl-CoA hydrolase activity (PubMed:19170545). kcat is 0.0076 s(-1) for crotonyl-CoA hydrolase activity (PubMed:19170545). kcat is 0.02 s(-1) for tiglyl-CoA hydrolase activity (PubMed:19170545). kcat is 0.036 s(-1) for n- propionyl-CoA hydrolase activity (PubMed:19170545). kcat is 0.054 s(- 1) for acetyl-CoA hydrolase activity (PubMed:19170545). kcat is 0.019 s(-1) for lauroyl-CoA hydrolase activity (PubMed:19170545). kcat is 0.02 s(-1) for myristoyl-CoA (at 25 degrees Celsius) hydrolase activity (PubMed:19170545). kcat is 0.07 s(-1) for myristoyl-CoA (at 37 degrees Celsius) hydrolase activity (PubMed:19170545). kcat is 0.0044 s(-1) for n-palmitoyl-CoA hydrolase activity (PubMed:19170545). kcat is 0.017 s(-1) for palmitoleoyl-CoA hydrolase activity (PubMed:19170545). kcat is 0.011 s(-1) for stearoyl-CoA hydrolase activity (PubMed:19170545). kcat is 0.011 s(-1) for oleoyl- CoA hydrolase activity (PubMed:19170545). kcat is 0.017 s(-1) for linoleoyl-CoA hydrolase activity (PubMed:19170545). kcat is 0.022 s(- 1) for arachidonoyl-CoA hydrolase activity (PubMed:19170545). kcat is 1.4 s(-1) for 3-hydroxyphenylacetyl-CoA hydrolase activity (PubMed:19170545). kcat is 0.19 s(-1) for 3,4-dihydroxyphenylacetyl- CoA hydrolase activity (PubMed:19170545). kcat is 0.19 s(-1) for 3,5- dihydroxyphenylacetyl-CoA hydrolase activity (PubMed:19170545). kcat is 0.14 s(-1) for 4-hydroxyphenylacetyl-CoA hydrolase activity (PubMed:19170545). kcat is 0.084 s(-1) for phenylacetyl-CoA hydrolase activity (PubMed:19170545). kcat is 0.011 s(-1) for 3-hydroxybenzoyl- CoA hydrolase activity (PubMed:19170545). kcat is 0.032 s(-1) for 4- chlorobenzoyl-CoA hydrolase activity (PubMed:19170545). kcat is 0.18 s(-1) for beta-hydroxybutyryl-CoA hydrolase activity (PubMed:19170545). kcat is 0.05 s(-1) for glutaryl-CoA hydrolase activity (PubMed:19170545). kcat is 0.079 s(-1) for 3-hydroxy-3- methylglutaryl-CoA hydrolase activity (PubMed:19170545). kcat is 0.066 s(-1) for malonyl-CoA hydrolase activity (PubMed:19170545). kcat is 0.083 s(-1) for methylmalonyl-CoA hydrolase activity (PubMed:19170545). {ECO:0000269|PubMed:19170545}; |
| Subunit: | |
Homotetramer (PubMed:16934754, PubMed:19170545). Interacts with PCTP (By similarity). {ECO:0000250|UniProtKB:Q9CQR4, ECO:0000269|PubMed:16934754, ECO:0000269|PubMed:19170545}. |
| Subcellular location: | |
Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q9CQR4}. Mitochondrion {ECO:0000250|UniProtKB:Q9CQR4}. Nucleus {ECO:0000250|UniProtKB:Q9CQR4}. Cytoplasm, cytoskeleton, spindle {ECO:0000250|UniProtKB:Q9CQR4}. Note=During interphase, found both in the nucleus and in the cytoplasm. At mitosis, localizes to the spindle. Colocalizes with tubulin. {ECO:0000250|UniProtKB:Q9CQR4}. |
| Similarity: | |
Belongs to the thioesterase PaaI family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.