 |
PDBsum entry 2erl
|
|
|
|
References listed in PDB file
|
 |
|
Key reference
|
|
|
Title
|
|
A challenging case for protein crystal structure determination: the mating pheromone er-1 from euplotes raikovi.
|
|
|
Authors
|
|
D.H.Anderson,
M.S.Weiss,
D.Eisenberg.
|
|
|
Ref.
|
|
Acta Crystallogr D Biol Crystallogr, 1996,
52,
469-480.
[DOI no: ]
|
|
|
PubMed id
|
|
|
|
|
Note In the PDB file this reference is
annotated as "TO BE PUBLISHED".
The citation details given above were identified by an automated
search of PubMed on title and author
names, giving a
perfect match.
|
|
|
|
Abstract
|
|
|
Four different phasing methods have been applied to the determination of the
crystal structure of the 40 amino-acid mating pheromone of the unicellular
ciliated protozoan Euplotes raikovi. The difficulties, failures and successes in
attempts to solve the structure by: (1) molecular replacement, (2) direct
phasing using the 'Shake and Bake' algorithm, (3) isomorphous replacement, and
(4) multiple-wavelength anomalous dispersion are described. The structure was
first solved by molecular replacement, and then was the first successful
structure determination by 'Shake and Bake' without the direct involvement of
its authors. A description of the current status of the high-resolution
refinement of the structure is also given. The model is refined against 1 A
resolution data to an R factor of 12.9%, and includes H atoms and discretely
disordered side chains.
|
|
|
|
|
|
Figure 4.
Fig. 4. Stereoview of all the
protein atoms in the model
of Er-l, with the anomalous
Fourier superimposed (con-
toured at +3or). There are
peaks only at the locations
of S atoms, showing that
the S atoms are correctly
placed. The view is similar
to that in Fig. l(a) of Weiss
et al.
(1995). This figure was
prepared with the program
FRODO
(Jones, 1978).
|
|
|
|
|
|
The above figure is
reprinted
by permission from the IUCr:
Acta Crystallogr D Biol Crystallogr
(1996,
52,
469-480)
copyright 1996.
|
|
|
Secondary reference #1
|
|
|
Title
|
|
A cooperative model for receptor recognition and cell adhesion: evidence from the molecular packing in the 1.6-A crystal structure of the pheromone er-1 from the ciliated protozoan euplotes raikovi.
|
|
|
Authors
|
|
M.S.Weiss,
D.H.Anderson,
S.Raffioni,
R.A.Bradshaw,
C.Ortenzi,
P.Luporini,
D.Eisenberg.
|
|
|
Ref.
|
|
Proc Natl Acad Sci U S A, 1995,
92,
10172-10176.
[DOI no: ]
|
|
|
PubMed id
|
|
|
|
|
|
|
|
|
Secondary reference #2
|
|
|
Title
|
|
The nmr solution structure of the pheromone er-1 from the ciliated protozoan euplotes raikovi.
|
|
|
Authors
|
|
S.Mronga,
P.Luginbühl,
L.R.Brown,
C.Ortenzi,
P.Luporini,
R.A.Bradshaw,
K.Wüthrich.
|
|
|
Ref.
|
|
Protein Sci, 1994,
3,
1527-1536.
[DOI no: ]
|
|
|
PubMed id
|
|
|
|
|
|
|
|
|
Secondary reference #3
|
|
|
Title
|
|
Crystallization of the euplotes raikovi mating pheromone er-1.
|
|
|
Authors
|
|
D.Anderson,
S.Raffioni,
P.Luporini,
R.A.Bradshaw,
D.Eisenberg.
|
|
|
Ref.
|
|
J Mol Biol, 1990,
216,
1-2.
|
|
|
PubMed id
|
|
|
|
|
|
|
|
|
Secondary reference #4
|
|
|
Title
|
|
Primary structure of the mating pheromone er-1 of the ciliate euplotes raikovi.
|
|
|
Authors
|
|
S.Raffioni,
P.Luporini,
B.T.Chait,
S.S.Disper,
R.A.Bradshaw.
|
|
|
Ref.
|
|
J Biol Chem, 1988,
263,
18152-18159.
|
|
|
PubMed id
|
|
|
|
|
|
|
|
|
|
|
|
|
