PDBsum entry 2ejm

Ligase

PDB id: 2ejm

Contents

Protein chain

99 a.a. *

* Residue conservation analysis

PDB id:

2ejm

Name:

Ligase

Title:

Solution structure of ruh-072, an apo-biotnyl domain form human acetyl coenzyme a carboxylase

Structure:

Methylcrotonoyl-coa carboxylase subunit alpha. Chain: a. Fragment: c-terminal domain of acetyl coa carboxylase. Synonym: 3-methylcrotonyl-coa carboxylase 1, mccase subunit alpha, 3- methylcrotonyl-coa:carbon dioxide ligase subunit alpha, 3- methylcrotonyl-coa carboxylase biotin-containing subunit. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: mccc1, mcca. Expressed in: escherichia coli. Expression_system_taxid: 562. Other_details: cell-free protein synthesis

NMR struc:

20 models

Authors:

A.Z.M.Ruhul Momen,H.Hirota,F.Hayashi,S.Yokoyama,Riken Structural Genomics/proteomics Initiative (Rsgi)

Key ref:

A.Z.M.Ruhul momen et al. Solution structure of ruh-072, An apo-Biotnyl domain form human acetyl coenzyme a carboxylase. To be published, .

Date:

19-Mar-07 Release date: 25-Sep-07

Protein chain

Q96RQ3  (MCCA_HUMAN) -  Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial from Homo sapiens

Seq: 725 a.a.

Struc: 99 a.a.*

* PDB and UniProt seqs differ at 6 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.6.4.1.4 - methylcrotonoyl-CoA carboxylase.
• Reaction: 3-methylbut-2-enoyl-CoA + hydrogencarbonate + ATP = 3-methyl-(2E)- glutaconyl-CoA + ADP + phosphate + H⁺
• Cofactor: Biotin

Molecule diagrams generated from .mol files obtained from the KEGG ftp site