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PDBsum entry 2ej9
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protein ligands links
Ligase PDB id
2ej9

 

 

 

 

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JSmol PyMol  
Contents
Protein chain
237 a.a. *
Ligands
BTN
Waters ×206
* Residue conservation analysis
PDB id:
2ej9
Name: Ligase
Title: Crystal structure of biotin protein ligase from methanococcus jannaschii
Structure: Putative biotin ligase. Chain: a. Engineered: yes
Source: Methanocaldococcus jannaschii. Organism_taxid: 2190. Gene: bpl. Expressed in: escherichia coli. Expression_system_taxid: 562.
Resolution:
2.00Å     R-factor:   0.216     R-free:   0.244
Authors: B.Bagautdinov,S.Bagautdinova,N.Kunishima,Riken Structural Genomics/proteomics Initiative (Rsgi)
Key ref: B.Bagautdinov et al. Crystal structure of biotin protein ligase from methanococcus jannaschii. To be published, .
Date:
16-Mar-07     Release date:   18-Mar-08    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q59014  (BPL_METJA) -  Putative biotin ligase from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
Seq:
Struc: 		237 a.a.
237 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.6.3.4.15  - biotin--[biotin carboxyl-carrier protein] ligase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: biotin + L-lysyl-[protein] + ATP = N6-biotinyl-L-lysyl-[protein] + AMP + diphosphate + H+
biotin
 + L-lysyl-[protein]
Bound ligand (Het Group name = BTN)
corresponds exactly 		+ ATP
 = N(6)-biotinyl-L-lysyl-[protein]
 + AMP
 + diphosphate
 + H(+)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 

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