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UniProt functional annotation for P22643 | ||
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| UniProt code: P22643. |
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| Organism: | |
Xanthobacter autotrophicus. |
| Taxonomy: | |
Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Xanthobacteraceae; Xanthobacter. |
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| Function: | |
Catalyzes hydrolytic cleavage of carbon-halogen bonds in halogenated aliphatic compounds, leading to the formation of the corresponding primary alcohols, halide ions and protons. Has a broad substrate specificity, which includes terminally mono- and di- chlorinated and brominated alkanes (up to C4 only). The highest activity was found with 1,2-dichloroethane, 1,3-dichloropropane, and 1,2-dibromoethane. |
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| Catalytic activity: | |
Reaction=1-haloalkane + H2O = a halide anion + a primary alcohol + H(+); Xref=Rhea:RHEA:19081, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15734, ChEBI:CHEBI:16042, ChEBI:CHEBI:18060; EC=3.8.1.5; |
| Catalytic activity: | |
Reaction=1,2-dichloroethane + H2O = 2-chloroethanol + chloride + H(+); Xref=Rhea:RHEA:25185, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17996, ChEBI:CHEBI:27789, ChEBI:CHEBI:28200; EC=3.8.1.5; |
| Activity regulation: | |
Inhibited by thiol reagents such as p- chloromercuribenzoate and iodoacetamide. |
| Biophysicochemical properties: | |
pH dependence: Optimum pH is 8.2.; Temperature dependence: Optimum temperature is 37 degrees Celsius.; |
| Pathway: | |
Xenobiotic degradation; 1,2-dichloroethane degradation; glycolate from 1,2-dichloroethane: step 1/4. |
| Subunit: | |
Monomer. |
| Similarity: | |
Belongs to the haloalkane dehalogenase family. Type 1 subfamily. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.