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UniProt functional annotation for P69441 | ||
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| UniProt codes: P69441, P05082. |
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| Organism: | |
Escherichia coli (strain K12). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
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| Function: | |
Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism. {ECO:0000255|HAMAP- Rule:MF_00235, ECO:0000269|PubMed:166976, ECO:0000269|PubMed:6243627}. |
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| Catalytic activity: | |
Reaction=AMP + ATP = 2 ADP; Xref=Rhea:RHEA:12973, ChEBI:CHEBI:30616, ChEBI:CHEBI:456215, ChEBI:CHEBI:456216; EC=2.7.4.3; Evidence={ECO:0000255|HAMAP-Rule:MF_00235, ECO:0000269|PubMed:10398370}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=51 uM for ATP (at pH 7.4 and 30 degrees Celsius) {ECO:0000269|PubMed:10398370}; KM=38 uM for AMP (at pH 7.4 and 30 degrees Celsius) {ECO:0000269|PubMed:10398370}; KM=92 uM for ADP (at pH 7.4 and 30 degrees Celsius) {ECO:0000269|PubMed:10398370}; Vmax=1020 umol/min/mg enzyme for the forward reaction (at pH 7.4 and 30 degrees Celsius) {ECO:0000269|PubMed:10398370}; Vmax=605 umol/min/mg enzyme for the reverse reaction (at pH 7.4 and 30 degrees Celsius) {ECO:0000269|PubMed:10398370}; Temperature dependence: Optimum temperature is 45 degrees Celsius. Thermostable. Is half- inactivated at 52 degrees Celsius. {ECO:0000269|PubMed:10398370}; |
| Pathway: | |
Purine metabolism; AMP biosynthesis via salvage pathway; AMP from ADP: step 1/1. {ECO:0000255|HAMAP-Rule:MF_00235}. |
| Subunit: | |
Monomer. {ECO:0000255|HAMAP-Rule:MF_00235, ECO:0000269|PubMed:16302237, ECO:0000269|PubMed:19805185, ECO:0000269|PubMed:8451239}. |
| Subcellular location: | |
Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00235}. |
| Domain: | |
Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis. {ECO:0000255|HAMAP-Rule:MF_00235, ECO:0000305|PubMed:8805521}. |
| Similarity: | |
Belongs to the adenylate kinase family. {ECO:0000255|HAMAP- Rule:MF_00235}. |
| Sequence caution: | |
Sequence=AAB40228.1; Type=Erroneous initiation; Evidence={ECO:0000305}; |
Annotations taken from UniProtKB at the EBI.