|
|
||
|
|
|
|
|
UniProt functional annotation for Q12051 | ||
|
|
|
|
|
|
||
| UniProt code: Q12051. |
|
||
| Organism: | |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). |
| Taxonomy: | |
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. |
|
||
|
||
| Function: | |
Catalyzes the trans-addition of the 3 molecules of IPP onto DMAPP to form geranylgeranyl pyrophosphate. Required for the membrane attachment of YPT1 and SEC4. May be involved in vesicle trafficking and protein sorting. {ECO:0000269|PubMed:15296494, ECO:0000269|PubMed:7665600}. |
|
||
| Catalytic activity: | |
Reaction=dimethylallyl diphosphate + isopentenyl diphosphate = (2E)- geranyl diphosphate + diphosphate; Xref=Rhea:RHEA:22408, ChEBI:CHEBI:33019, ChEBI:CHEBI:57623, ChEBI:CHEBI:58057, ChEBI:CHEBI:128769; EC=2.5.1.1; |
| Catalytic activity: | |
Reaction=(2E)-geranyl diphosphate + isopentenyl diphosphate = (2E,6E)- farnesyl diphosphate + diphosphate; Xref=Rhea:RHEA:19361, ChEBI:CHEBI:33019, ChEBI:CHEBI:58057, ChEBI:CHEBI:128769, ChEBI:CHEBI:175763; EC=2.5.1.10; |
| Catalytic activity: | |
Reaction=(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate = (2E,6E,10E)-geranylgeranyl diphosphate + diphosphate; Xref=Rhea:RHEA:17653, ChEBI:CHEBI:33019, ChEBI:CHEBI:58756, ChEBI:CHEBI:128769, ChEBI:CHEBI:175763; EC=2.5.1.29; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:17535895}; Note=Binds 2 Mg(2+) ions per subunit. {ECO:0000269|PubMed:17535895}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=3.2 uM for farnesyl diphosphate; KM=0.8 uM for isopentenyl diphosphate; |
| Pathway: | |
Isoprenoid biosynthesis; farnesyl diphosphate biosynthesis; farnesyl diphosphate from geranyl diphosphate and isopentenyl diphosphate: step 1/1. |
| Pathway: | |
Isoprenoid biosynthesis; geranyl diphosphate biosynthesis; geranyl diphosphate from dimethylallyl diphosphate and isopentenyl diphosphate: step 1/1. |
| Pathway: | |
Isoprenoid biosynthesis; geranylgeranyl diphosphate biosynthesis; geranylgeranyl diphosphate from farnesyl diphosphate and isopentenyl diphosphate: step 1/1. |
| Subcellular location: | |
Cytoplasm {ECO:0000250}. |
| Miscellaneous: | |
Present with 2840 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}. |
| Similarity: | |
Belongs to the FPP/GGPP synthase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.