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UniProt functional annotation for O67728 | ||
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| UniProt code: O67728. |
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| Organism: | |
Aquifex aeolicus (strain VF5). |
| Taxonomy: | |
Bacteria; Aquificae; Aquificales; Aquificaceae; Aquifex. |
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| Function: | |
Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine. {ECO:0000255|HAMAP-Rule:MF_01161}. |
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| Catalytic activity: | |
Reaction=ATP + cytidine(34) in tRNA(Ile2) + L-lysine = AMP + diphosphate + H(+) + lysidine(34) in tRNA(Ile2); Xref=Rhea:RHEA:43744, Rhea:RHEA-COMP:10625, Rhea:RHEA-COMP:10670, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:32551, ChEBI:CHEBI:33019, ChEBI:CHEBI:82748, ChEBI:CHEBI:83665, ChEBI:CHEBI:456215; EC=6.3.4.19; Evidence={ECO:0000255|HAMAP- Rule:MF_01161}; |
| Subcellular location: | |
Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01161}. |
| Domain: | |
The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding. |
| Similarity: | |
Belongs to the tRNA(Ile)-lysidine synthase family. {ECO:0000255|HAMAP-Rule:MF_01161}. |
Annotations taken from UniProtKB at the EBI.