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UniProt functional annotation for Q71EW5 | ||
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| UniProt code: Q71EW5. |
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| Organism: | |
Pelobacter acetylenicus. |
| Taxonomy: | |
Bacteria; Proteobacteria; Deltaproteobacteria; Desulfuromonadales; Syntrophotaleaceae; Syntrophotalea. |
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| Function: | |
Catalyzes the hydration of acetylene to form acetaldehyde. Ethylene cannot act as a substrate. {ECO:0000269|PubMed:7592321}. |
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| Catalytic activity: | |
Reaction=acetaldehyde = acetylene + H2O; Xref=Rhea:RHEA:17885, ChEBI:CHEBI:15343, ChEBI:CHEBI:15377, ChEBI:CHEBI:27518; EC=4.2.1.112; Evidence={ECO:0000269|PubMed:7592321}; |
| Cofactor: | |
Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000269|PubMed:17360611}; Note=Binds 1 [4Fe-4S] cluster. {ECO:0000269|PubMed:17360611}; |
| Cofactor: | |
Name=W-bis(molybdopterin guanine dinucleotide); Xref=ChEBI:CHEBI:60537; Evidence={ECO:0000269|PubMed:17360611}; Note=Binds 1 W-bis(molybdopterin guanine dinucleotide) (W-bis-MGD) cofactor per subunit. {ECO:0000269|PubMed:17360611}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=14 uM for acetylene {ECO:0000269|PubMed:7592321}; Vmax=69 umol/min/mg enzyme {ECO:0000269|PubMed:7592321}; pH dependence: Optimum pH is 6.0-6.5. {ECO:0000269|PubMed:7592321}; Temperature dependence: Optimum temperature is 50 degrees Celsius. {ECO:0000269|PubMed:7592321}; |
| Subunit: | |
Monomer. {ECO:0000269|PubMed:17360611, ECO:0000269|PubMed:7592321}. |
| Miscellaneous: | |
The tungsten center binds a water molecule that is activated by Asp-13 residue, enabling it to attack acetylene bound in a distinct hydrophobic pocket. {ECO:0000305|PubMed:17360611}. |
| Similarity: | |
Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.