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PDBsum entry 2dov
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Plant protein, sugar binding protein
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PDB id
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2dov
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References listed in PDB file
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Key reference
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Title
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Cdna sequences, Maldi-Tof analyses, And molecular modelling of barley pr-5 proteins.
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Authors
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E.Reiss,
B.Schlesier,
W.Brandt.
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Ref.
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Phytochemistry, 2006,
67,
1856-1864.
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PubMed id
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Abstract
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Barley plants are known to produce various PR-5 proteins. Transcripts encoding
eight different barley PR-5 proteins (TLPs 1-8, TLP for thaumatin-like protein)
were identified and cloned - seven from infected leaves and one from developing
grains. Here, we describe the cDNA sequences of four of these TLP isoforms.
Moreover, the TLPs from the infected leaves (TLPs 1, 2, and TLPs 4-8) were
subjected to MALDI-TOF mass spectrometric measurements that resulted in protein
fragments consistent with their deduced peptide sequences. Multiple sequence
alignment analysis revealed that the TLPs in barley fall into two groups:
long-chain proteins (TLPs 5-8) having 16 cysteine residues and short-chain
proteins (TLPs 1-4) with only 10 cysteine residues. Finally, modelling
experiments highlighted the effects of sequence differences between the TLP
isoforms in terms of their secondary structures and their molecular
electrostatic potentials. We propose that these sequence differences have
implications for the target preferences of the different isomers.
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