PDBsum entry 2dld

Oxidoreductase (choh(d)-NAD+(a))

PDB id: 2dld

Contents

Protein chains

337 a.a. *

Ligands

NAI

OXM

NAI-OXM

* Residue conservation analysis

PDB id:

2dld

Name:

Oxidoreductase (choh(d)-NAD+(a))

Title:

D-lactate dehydrogenase complexed with nadh and oxamate

Structure:

D-lactate dehydrogenase. Chain: a, b. Synonym: r-lactate dehydrogenase. Other_details: crystallized in 11% peg 4000, 5 mm bis-tris ph 6.8, 75 mg/ml enzyme, 3 mm nadh, 10 mm oxamate

Source:

Lactobacillus helveticus. Organism_taxid: 1587

Biol. unit:

Dimer (from PQS)

Resolution:

2.70Å R-factor: 0.195 R-free: 0.297

Authors:

C.R.Dunn,J.J.Holbrook

Key ref:

N.Bernard et al. Dehydrogenases engineering to correct substrate inhibition in a commercial dehydrogenase. To be published, .

Date:

28-Oct-95 Release date: 14-Mar-96

Protein chains

P30901  (LDHD_LACHE) -  D-lactate dehydrogenase from Lactobacillus helveticus

Seq: 337 a.a.

Struc: 337 a.a.

Enzyme reactions

• Enzyme class: E.C.1.1.1.28 - D-lactate dehydrogenase.
• Reaction: (R)-lactate + NAD⁺ = pyruvate + NADH + H⁺

(R)-lactate (Bound ligand (Het Group name = OXM) matches with 71.43% similarity) + NAD(+) (Bound ligand (Het Group name = NAI) corresponds exactly) = pyruvate + NADH + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site