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PDBsum entry 2dhe
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References listed in PDB file
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Key reference
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Title
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Crystallographic analysis of the catalytic mechanism of haloalkane dehalogenase.
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Authors
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K.H.Verschueren,
F.Seljée,
H.J.Rozeboom,
K.H.Kalk,
B.W.Dijkstra.
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Ref.
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Nature, 1993,
363,
693-698.
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PubMed id
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Abstract
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Crystal structures of haloalkane dehalogenase were determined in the presence of
the substrate 1,2-dichloroethane. At pH 5 and 4 degrees C, substrate is bound in
the active site without being converted; warming to room temperature causes the
substrate's carbon-chlorine bond to be broken, producing a chloride ion with
concomitant alkylation of the active-site residue Asp124. At pH 6 and room
temperature the alkylated enzyme is hydrolysed by a water molecule activated by
the His289-Asp260 pair in the active site. These results show that catalysis by
the dehalogenase proceeds by a two-step mechanism involving an ester
intermediate covalently bound at Asp124.
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Secondary reference #1
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Title
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Crystal structure of haloalkane dehalogenase: an enzyme to detoxify halogenated alkanes.
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Authors
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S.M.Franken,
H.J.Rozeboom,
K.H.Kalk,
B.W.Dijkstra.
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Ref.
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Embo J, 1991,
10,
1297-1302.
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PubMed id
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Secondary reference #2
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Title
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Crystallization of haloalkane dehalogenase from xanthobacter autotrophicus gj10.
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Authors
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H.J.Rozeboom,
J.Kingma,
D.B.Janssen,
B.W.Dijkstra.
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Ref.
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J Mol Biol, 1988,
200,
611-612.
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PubMed id
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