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PDBsum entry 2dgj
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Cell adhesion
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PDB id
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2dgj
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References listed in PDB file
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Key reference
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Title
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A helical string of alternately connected three-Helix bundles for the cell wall-Associated adhesion protein ebh from staphylococcus aureus.
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Authors
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Y.Tanaka,
S.Sakamoto,
M.Kuroda,
S.Goda,
Y.G.Gao,
K.Tsumoto,
Y.Hiragi,
M.Yao,
N.Watanabe,
T.Ohta,
I.Tanaka.
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Ref.
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Structure, 2008,
16,
488-496.
[DOI no: ]
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PubMed id
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Abstract
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The 1.1 MDa cell-wall-associated adhesion protein of staphylococci, Ebh,
consists of several distinct regions, including a large central region with 52
imperfect repeats of 126 amino acid residues. We investigated the structure of
this giant molecule by X-ray crystallography, circular dichroism (CD)
spectrometry, and small-angle X-ray scattering (SAXS). The crystal structure of
two repeats showed that each repeat consists of two distinct three-helix
bundles, and two such repeats are connected along the long axis, resulting in a
rod-like structure that is 120 A in length. CD and SAXS analyses of the samples
with longer repeats suggested that each repeat has an identical structure, and
that such repeats are connected tandemly to form a rod-like structure in
solution, the length of which increased proportionately with the number of
repeating units. On the basis of these results, it was proposed that Ebh is a
320 nm rod-like molecule with some plasticity at module junctions.
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Figure 1.
Figure 1. Crystal Structure of EbhA-R7-R8
(A) Ribbon
diagram of EbhA-R7-R8. The ribbon model is colored according to
the sequence, from blue at the N terminus to red at the C
terminus. The repeating units are indicated.
(B)
Topological diagram of EbhA-R7-R8. Squares indicate α helices.
Colors correspond to those in (A).
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Figure 6.
Figure 6. Low-Resolution Structures of EbhA-R7, EbhA-R7-R8,
and EbhA-R5-R8
(A–C) Calculated low-resolution structures
are shown as green balls. Crystal structures of (A) EbhA-R7 and
(B) EbhA-R7-R8 superposed on those of low-resolution structures
are also shown as red ribbon diagrams. The structure of (C)
EbhA-R5-R8 inferred from the crystal structure of EbhA-R7-R8 is
also shown as a red ribbon diagram. The lengths of
low-resolution structures and structures deduced from the
crystal structure of EbhA-R7-R8 are also indicated as green and
red letters, respectively.
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The above figures are
reprinted
by permission from Cell Press:
Structure
(2008,
16,
488-496)
copyright 2008.
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